1.14.13.148: trimethylamine monooxygenase

This is an abbreviated version!
For detailed information about trimethylamine monooxygenase, go to the full flat file.

Reaction

Synonyms

dimethyl sulfoxide/trimethylamine N-oxide reductase, EC 1.14.13.8, FAD-containing monooxygenase, flavin containing monooxygenase 3, flavin monooxygenase 3, flavin-containing monooxygenase, flavin-containing monooxygenase 3, FMO, FMO3, FMO4, PB7211, TMA monooxygenase, TMA N-oxygenase, TMM

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.148 trimethylamine monooxygenase

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Results	in table
4	Activating Compound
68	AA Sequence
12	Cloned(Commentary)
46	Cofactor
1	Crystallization (Commentary)
29	Disease/ Diagnostics
4	Expression
8	General Information
1	General Stability
22	Inhibitors
3	kcat/KM [mM/s]
46	KM Value [mM]
12	Localization
2	Metals/Ions
6	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
41	Organism
5	Pathway
4	pH Optimum
2	pH Range
1	pH Stability
1	Posttranslational Modification
24	Protein Variants
3	Purification (Commentary)
1	Reaction
3	Reaction Type
37	Reference
20	Source Tissue
5	Specific Activity [micromol/min/mg]
67	Substrates and Products (Substrate)
5	Subunits
77	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
4	Temperature Range [°C]
5	Temperature Stability [°C]
15	Turnover Number [1/s]

KM Value

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KM Value on EC 1.14.13.148 - trimethylamine monooxygenase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.1116

5-{[3-(dimethylamino)propyl]amino}-8-hydroxy-6H-[1,2,3]triazolo[4,5,1-de]acridin-6-one

Homo sapiens

P31513

isozyme FMO3, in 0.1 M potassium phosphate buffer (pH 8.4) at 37°C

718455

0.1624

5-{[3-(dimethylamino)propyl]amino}-8-methoxy-6H-[1,2,3]triazolo[4,5,1-de]acridin-6-one

Homo sapiens

P31513

isozyme FMO3, in 0.1 M potassium phosphate buffer (pH 8.4) at 37°C

718455

0.0186 - 0.056

Benzydamine

4 entries

3.139

cysteamine

Methylocella silvestris

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.25

Dimethyl sulfide

Candidatus Pelagibacter sp. HTCC7211

B6BT33

wild-type, pH 7, 25°C

764744

0.0357

dimethylaniline

Methylocella silvestris

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.0103

Dimethylsulfide

Methylocella silvestris

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

3.575

DMSO

Methylocella silvestris

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.114 - 0.336

Ethionamide

2 entries

0.0282 - 0.116

Methimazole

7 entries

0.0003 - 0.139

N,N,N-trimethylamine

9 entries

0.0897 - 0.181

N,N-dimethylamine

2 entries

2

NADH

Aminobacter aminovorans

-

pH and temperature not specified in the publication

717207

0.0008 - 0.1348

NADPH

11 entries

0.022 - 0.0693

sulindac sulfide

3 entries

0.0186

Benzydamine

Canis lupus familiaris

-

in in 0.1 M tricine buffer, pH 7.4, at 37°C

703411

0.0426

Benzydamine

Homo sapiens

-

in in 0.1 M tricine buffer, pH 7.4, at 37°C

703411

0.045

Benzydamine

Homo sapiens

-

truncated recombinant enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C

717229

0.056

Benzydamine

Homo sapiens

-

wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C

717229

0.114

Ethionamide

Mus musculus

P97501

isozyme FMO3, at pH 9.5 and 37°C

706824

0.336

Ethionamide

Homo sapiens

P31513

isozyme FMO3, at pH 9.5 and 37°C

706824

0.0282

Methimazole

Methylocella silvestris

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.0285

Methimazole

Homo sapiens

-

mutant enzyme, at pH and 37°C

348510

0.03

Methimazole

Oryctolagus cuniculus

P32417, P36367

isoform FMO3, at pH 8.4, temperature not specified in the publication

717752

0.03

Methimazole

Homo sapiens

-

native enzyme, at pH and 37°C

348510

0.033

Methimazole

Homo sapiens

-

wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C

717229

0.035

Methimazole

Homo sapiens

-

truncated recombinant enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C

717229

0.116

Methimazole

Candidatus Pelagibacter sp. HTCC7211

B6BT33

wild-type, pH 7, 25°C

764744

0.0003

N,N,N-trimethylamine

Calanus finmarchicus

-

in potassium diphosphate buffer (pH 7.7), at 14°C

717521

0.0094

N,N,N-trimethylamine

Methylocella silvestris

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.013 - 0.0548

N,N,N-trimethylamine

Homo sapiens

-

the Km for isoform FMO3 determined in human liver microsomes ranges from 0.013.to 0.0548 mM, at pH 7.4 and 22°C

717226

0.0208

N,N,N-trimethylamine

Ruegeria pomeroyi

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.0216

N,N,N-trimethylamine

Roseovarius sp.

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.0275

N,N,N-trimethylamine

Candidatus Pelagibacter ubique

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.028

N,N,N-trimethylamine

Homo sapiens

-

recombinant isoform FMO3, at pH 7.4 and 22°C

717226

0.0285

N,N,N-trimethylamine

Candidatus Pelagibacter ubique

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.139

N,N,N-trimethylamine

Candidatus Pelagibacter sp. HTCC7211

B6BT33

wild-type, pH 7, 25°C

764744

0.0897

N,N-dimethylamine

Methylocella silvestris

-

recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

718325

0.181

N,N-dimethylamine

Candidatus Pelagibacter sp. HTCC7211

B6BT33

wild-type, pH 7, 25°C

764744

0.0008

NADPH

Calanus finmarchicus

-

in potassium diphosphate buffer (pH 7.7), at 14°C

717521

0.0097

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

mutant D314E, pH 7, 25°C

764744

0.0128

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

wild-type, pH 7, 25°C

764744

0.016

NADPH

Aminobacter aminovorans

-

pH and temperature not specified in the publication

717207

0.032

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

mutant D314A, pH 7, 25°C

764744

0.0341

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

mutant N72A, pH 7, 25°C

764744

0.0412

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

mutant H227A, pH 7, 25°C

764744

0.0449

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

mutant R409A, pH 7, 25°C

764744

0.0611

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

mutant N288A, pH 7, 25°C

764744

0.1018

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

mutant R226A, pH 7, 25°C

764744

0.1348

NADPH

Candidatus Pelagibacter sp. HTCC7211

B6BT33

mutant S203A, pH 7, 25°C

764744

0.022

sulindac sulfide

Homo sapiens

-

truncated recombinant enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C

717229

0.025

sulindac sulfide

Homo sapiens

-

wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C

717229

0.0693

sulindac sulfide

Homo sapiens

-

in in 0.1 M tricine buffer, pH 9.0, at 37°C

703411