1.14.13.135: 1-hydroxy-2-naphthoate hydroxylase

This is an abbreviated version!
For detailed information about 1-hydroxy-2-naphthoate hydroxylase, go to the full flat file.

Reaction

1-hydroxy-2-naphthoate
+
NAD(P)H
+
H+
+
O2
=
1,2-dihydroxynaphthalene
+
NAD(P)+
+
H2O
+
CO2

Synonyms

1-hydroxy-2-naphthoic acid hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.135 1-hydroxy-2-naphthoate hydroxylase

Cofactor

Cofactor on EC 1.14.13.135 - 1-hydroxy-2-naphthoate hydroxylase

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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
holoenzyme contains FAD. Km is 0.0047 mM. Preparation of the apoenzyme by the acid-ammonium sulfate, at 2 M, dialysis method, the apoenzyme is colorless, inactive and loses the characteristic flavin absorption spectra but regains about 90% of maximal activity when reconstituted with FAD
NADH
-
Vmax/Km is similar for NADPH and NADH
NADPH
-
Vmax/Km is similar for NADPH and NADH
additional information
-
FMN is a poor substitute for FAD
-