1.14.13.114: 6-hydroxynicotinate 3-monooxygenase

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For detailed information about 6-hydroxynicotinate 3-monooxygenase, go to the full flat file.

Reaction

Synonyms

6-hydroxynicotinic acid 3-monooxygenase, 6HNA monooxygenase, BB1770, BbNicC, NicC, PpNicC

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.114 6-hydroxynicotinate 3-monooxygenase

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Results	in table
509	AA Sequence
2	Application
6	Cloned(Commentary)
11	Cofactor
1	Crystallization (Commentary)
21	General Information
11	Inhibitors
18	kcat/KM [mM/s]
3	Ki Value [mM]
23	KM Value [mM]
2	Localization
2	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
53	Organism
4	Pathway
1	PDB ID
4	pH Optimum
1	pH Range
1	pH Stability
23	Protein Variants
4	Purification (Commentary)
8	Reaction
12	Reference
1	Specific Activity [micromol/min/mg]
25	Substrates and Products (Substrate)
7	Subunits
23	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Stability [°C]
18	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.14.13.114 - 6-hydroxynicotinate 3-monooxygenase

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purified recombinant His-tagged selenomethionine-labeled enzyme, hanging drop vapor diffusion method, mixing of 0.0015 ml of protein solution with 0.0015 ml of reservoir solution containing 0.1 M Tris-HCl, pH 7.7-8.3, 27-30% PEG 4000, and 0.2 M magnesium chloride hexahydrate, 18°C, 2-3 days, method optimization, X-ray diffraction structure determination and analysis using SAD phasing at 2.1 A resolution

Pseudomonas putida

Q88FY2

744353