Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.13.111: methanesulfonate monooxygenase (NADH)

This is an abbreviated version!
For detailed information about methanesulfonate monooxygenase (NADH), go to the full flat file.

Word Map on EC 1.14.13.111

Reaction

methanesulfonate
+
NADH
 +
H+
 +
O2
=
formaldehyde
 +
NAD+
 +
sulfite
 +
H2O

Synonyms

EC 1.14.14.6, methanesulfonate monooxygenase, methanesulfonic acid monooxygenase, MSA monooxygenase, MSAMO, Msm, MsmA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.111 methanesulfonate monooxygenase (NADH)

Cloned

Cloned on EC 1.14.13.111 - methanesulfonate monooxygenase (NADH)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the reductase encoded by the msmD gene. Cloning and overexpression of the msmD gene, encoding the reductase component, as a GST fusion protein results in the expression of a 65000 Da polypeptide matching the size of the GST protein plus the reductase protein when induced with isopropyl thio-beta-D-galactoside. The fusion between the two proteins is unstable, and purification by affinity chromatography is not possible
-
expression in Escherichia coli. The four polypeptides comprising MSAMO are the products of the coordinated expression of an operon (msmABCD)
-
gene encoding the electron transfer protein (MsmC) of methanesulfonic acid monooxygenase
-
gene msmA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha
msm gene cluster. Development of functional gene probes centered around the unique Rieske center encoding region of msmA which can be used to detect the presence of methanesulfonate-utilizing bacteria in the environment
-