1.14.13.101: senecionine N-oxygenase

This is an abbreviated version!
For detailed information about senecionine N-oxygenase, go to the full flat file.

Reaction

Synonyms

flavin-dependent monooxygenase, fmoa, GgPNO, oxygenase, senecionine N-, PA N-oxygenase, PNO, pyrrolizidine alkaloid N-oxygenase, senecionine monooxygenase (N-oxide-forming), senecionine N-oxygenase, SNO, TjSNO, ZvFMOa, ZvPNO

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.101 senecionine N-oxygenase

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Results	in table
3	Activating Compound
6	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
10	Cofactor
2	Crystallization (Commentary)
4	General Information
1	Inhibitors
5	kcat/KM [mM/s]
15	KM Value [mM]
3	Localization
4	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
16	Organism
3	Pathway
5	pH Optimum
1	pI Value
7	Protein Variants
5	Purification (Commentary)
1	Reaction
9	Reaction Type
15	Reference
13	Source Tissue
2	Specific Activity [micromol/min/mg]
78	Substrates and Products (Substrate)
4	Subunits
18	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.101 - senecionine N-oxygenase

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Tyria jacobaeae

651040

Ni2+-NTA agarose column chromatography

Zonocerus variegatus

728567

partial

Crotalaria scassellatii

689412

recombinant His6-tagged isozyme ZvPNO from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Zonocerus variegatus

L0N8F3, L0N8S9

763839

recombinant His6-tagged wild-type and mutant isozymes ZvFMOa from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Zonocerus variegatus

L0N8F3, L0N8S9

763839