1.14.12.11: toluene dioxygenase

This is an abbreviated version!
For detailed information about toluene dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.11

Reaction

Toluene
+
NADH
 +
H+
 +
O2
=
(1S,2R)-3-Methylcyclohexa-3,5-diene-1,2-diol
 +
NAD+

Synonyms

ISPTOD, ISPTOL, More, oxygenase, toluene 2,3-di-, oxygenaseTOL, TDO, Tod, todC1C2BA, toluene 2,3-dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.11 toluene dioxygenase

Storage Stability

Storage Stability on EC 1.14.12.11 - toluene dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, ferredoxinTOL component of the toluene dioxygenase, stable for over 10 weeks
-
-20°C, purified NADH-ferredoxinTOL reductase component is stable for two weeks
-
0-4°C, ferredoxinTOL component of the toluene dioxygenase, stable for up to 72 h
-
0-4°C, purified NADH-ferredoxinTOL reductase component is stable for up to 30 h
-