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1.14.11.9: flavanone 3-dioxygenase

This is an abbreviated version!
For detailed information about flavanone 3-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.9

Reaction

a (2S)-flavan-4-one
+
2-oxoglutarate
 +
O2
=
a (2R,3R)-dihydroflavonol
 +
succinate
 +
CO2

Synonyms

(2S)-flavanone 3-hydroxylase, AaF3H, AcF3H, BnF3H, CsF3H, CtF3H, F3H, F3H protein, F3H1, F3H2, FHT, FHTPH, flavanone 3-dioxygenase, flavanone 3-hydroxylase, flavanone 3beta-hydroxylase, flavanone synthase I, flavanone-3-hydroxylase, FLS1, FLS2, FS I, LcF3H, naringenin 3-dioxygenase, naringenin,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating), oxygenase, flavanone 3-di-, PeF3H, PgF3H, PnF3H, RtF3H1, RtF3H2, VcF3H

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.9 flavanone 3-dioxygenase

Cloned

Cloned on EC 1.14.11.9 - flavanone 3-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the pGEM T-easy plasmid vector and transformed into DH5alpha Escherichia coli competent cells
enzyme expression analysis by real-time RT-PCR
-
expressed in Escherichia coli BL21Star and Rosetta(DE3) cells
-
expressed in Escherichia coli strain BLR (DE3)
expressed in yeast strain InvSc1
expression as glutathione S-transferase fusion protein
expression in a reticulocyte system
expression in Escherichia coli
expression in Escherichia coli JM109
expression in Escherichia coli Transetta (DE3)
expression in Saccharomyces cerevisiae
-
expression in wheat-rye hybrids
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
gene CtF3H, DNA and amino acid sequence determination and analysis, phylogenic analysis, quantitative real-time PCR enzyme expression analysis, functional recombinant expression of MBP-tagged enzyme in Escherichia coli BL21(DE3)pLysS from vector pMAL-C5x leading to production of dihydrokaempferol when naringenin is available the substrate. Recombinant expression of GFP-tagged enzyme in onion cells via transfromation of Agrobacterium tumefaciens strain GV3101, where it is localized both in the nucleus and in the cytosol
gene F3H cloned from petals, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21
gene F3H, DNA and amino acid sequence determination and analysis, phylogenetic tree, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, quantitative real-time RT-PCR expression analysis
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
gene F3H, functional recombinant expression of codon-optimized enzyme in Corynebacterium glutamicum strain DelAro4, coexpression with gene FLS encoding flavonol synthase from Populus deltoides. Coexpression fo genes F3H and FLS both from Petunia hybrida is not successful
gene F3H-B1 and a fourth gene F3H-B2, a nonhomologous duplication of F3H-B1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
gene F3H-D1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
gene F3H1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses
gene F3H2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses
gene F3H2, genotyping in Glycine max cultivars and accessions, phylogenetic analysis
-
gene F3H2, genotyping in Glycine soja cultivars and accessions, phylogenetic analysis
-
gene F3HA1, DNA and amiino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, a recognition site of bZIP-type transcription factor (bZIP911) occurs in the promoter of F3H-A1
gene fht, transcription profiling and quantitative real-time PCR expression analysis
-
gene LcF3H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21(DE3), recombinant overexpression in leaves of Nicotiana tabacum cv. Xanthinc via transformation with Agrobacterium tumefaciens strain EHA105, overexpression of heterogenous LcF3H enhances the expression levels of downstream genes but does not cause alteration in the expression of upstream genes including endogenous F3H of Nicotiana tabacum. The enzyme's overexpression does cause a significant increase in the content of three major flavan-3-ols, catechin, epicatechin and epigallocatechin
gene PeF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli tsrain M15 pREP-4
gene PnF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Arabidopsis thaliana ecotype Col-0 via Agrobacterium tumefaciens-mediated transformation conferring tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
-
gene VcF3H, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR enzyme expression analysis, recombinant expression in Arabidopsis thaliana increasing the anthocyanin content in leaves without increasing the flavonol content
genes SbF3H1 and SbF3H2, semi-quantitative RT-PCR expression analysis of flavonoid structural genes in sorghum seedlings, overview. Complementation of Arabidopsis thaliana flavonoid mutants by genes SbF3H1 and SbF3H2, complementation analysis, overview
overexpression of the Camellia sinensis flavanone 3-hydroxylase gene in leaves of transgenic Nicotiana tabacum cv. Xanthi confers tolerance to salt stress and pathogenic fungus Alternaria solani, transformation via Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA-F3H. A decrease in pectin methyl esterase (PME) activity and increase in pectin methyl esterification is also observed in CsF3H transgenic tobacco plants. Transcript expression analysis, and analysis of trangenic PME activity in flavonoid (epigallocatechin)-exposed and -unexposed seedlings, and in seedlings exposed to salt
wild-type and mutant enzymes, expression in Escherichia coli
-