1.14.11.8: trimethyllysine dioxygenase
This is an abbreviated version!
For detailed information about trimethyllysine dioxygenase, go to the full flat file.
Word Map on EC 1.14.11.8
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1.14.11.8
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carnitine
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gamma-butyrobetaine
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autism
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inborn
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proliferator-activated
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hydroxymethyltransferase
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transporter-2
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aldolase
- 1.14.11.8
- carnitine
- gamma-butyrobetaine
-
autism
-
inborn
-
proliferator-activated
- hydroxymethyltransferase
-
transporter-2
- aldolase
Reaction
Synonyms
6-N-trimethyllysine dioxygenase, epsilon-N-trimethyllysine hydroxylase, epsilon-trimethyllysine 2-oxoglutarate dioxygenase, eta-N-trimethyllysine hydroxylase, Nepsilon-trimethyllysine hydroxylase, oxygenase, trimethyllysine di-, TML dioxygenase, TML hydroxylase, TML-alpha-ketoglutarate dioxygenase, TMLD, TMLH, TMLH-a, TMLH-b, trimethyllysine alpha-ketoglutarate dioxygenase, trimethyllysine hydroxylase
ECTree
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Cloned
Cloned on EC 1.14.11.8 - trimethyllysine dioxygenase
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expressed in COS-1 cells
gene TMLH, recombinant overexpression of active and stable TMLH as a His-tagged maltose-binding protein fusion in Escherichia coli strain BL21-AI. Coexpression of the enzyme with chaperones GroES/EL increases the expression level by one order of magnitude
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert