Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.11.66: [histone H3]-trimethyl-L-lysine9 demethylase

This is an abbreviated version!
For detailed information about [histone H3]-trimethyl-L-lysine9 demethylase, go to the full flat file.

Word Map on EC 1.14.11.66

Reaction

a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
+ 2 2-oxoglutarate + 2 O2 =
a [histone H3]-N6-methyl-L-lysine9
 + 2 succinate + 2 formaldehyde + 2 CO2

Synonyms

5qNCA, AN1060, BHC110, CG15835, CG33182, Dmel\Kdm4A, GASC1, H3K9 demethylase, H3K9 trimethyl demethylase, H3K9-specific demethylase, H3K9/36me3 lysine demethylase, H3K9me2/3 demethylase, H3K9Me3 demethylase, H3K9me3 histone demethylase, H3K9me3-specific demethylase, histone demethylase, histone demethylase JmjD2A, histone demethylase JMJD2A/KDM4A, histone demethylase JMJD2B, histone H3 demethylase, histone H3 Lys 9 demethylase, histone H3K9 demethylase, histone H3K9/H3K36 trimethyldemethylase, histone lysine demethylase, histone-3 lysine-9 di-/tri-methyl demethylase, JDHM3A, JHDM2A, JHDM2B, JHDM3A, JmjC histone lysine demethylase, JmjC-domain-containing histone demethylase 3A, JMJD1A, JMJD1B, JMJD2, JMJD2(1), JMJD2(2), JMJD2A, JMJD2A demethylase, JMJD2A/KDM4A, JMJD2B, JMJD2b histone demethylase, JMJD2C, JMJD2D, jumonji C-domaincontaining histone demethylase 3A, jumonji domain containing 2A, Jumonji domaincontaining demethylase, JumonjiC-domain-containing histone demethylase, JumonjiD2A, KDM1, KDM2B, Kdm3a, Kdm3b, KDM4, KDM4A, KDM4A lysine demethylase, KDM4A/JMJD2A, KDM4B, KDM4B/JMJD2B histone demethylase, Kdm4c, KDM4D, KDM4E, KdmA, KIAA0601, LD33386, LSD1, LSD1 demethylase, LSD1 H3 demethylase, lysine-specific demethylase 1, lysine-specific demethylase 4A, lysine-specific demethylase 4B, lysine-specific demethylase 4C, lysine-specific demethylase 4D, More, testis-enriched histone demethylase, tridemethylase of H3K9, trimethylated histone H3-lysine 9-specific demethylase, trimethyllysine-specific histone demethylase, trimethyllysine-specific JmjC HDM, [histone H3]-lysine-9 demethylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.66 [histone H3]-trimethyl-L-lysine9 demethylase

Cloned

Cloned on EC 1.14.11.66 - [histone H3]-trimethyl-L-lysine9 demethylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in NIH-3T3 cells and early mouse embryos
-
expressed in Sf9 insect cells
-
expression of full-length GFP-tagged JMJD2b-GFP-1086 and truncated JMJD2b forms in mouse fibroblasts and in Escherichia coli strain DH5alpha
-
expression of functional doxycycline-inducible EGFP-KDM4B in U2-OSTetON cell line
-
expression of GFP-tagged JmjD2A under the control beta-actin promoter in chicken fibroblasts
-
expression of N-terminally His-tagged KDM4A in Escherichia coli
expression of N-terminally His-tagged KDM4B in Escherichia coli
expression of N-terminally His-tagged KDM4C in Escherichia coli
expression of N-terminally His-tagged KDM4D in Escherichia coli
expression of N-terminally His-tagged KDM4E in Escherichia coli
gene CG15835 or Kdm4A, sequence comparisons, recombinant overexpression of FLAG-tagged wild-type and mutant enzymes in S2 cells
gene CG33182, sequence comparisons, recombinant overexpression of FLAG-tagged enzyme in S2 cells
gene JMJ706, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as GST-fusion protein
gene KDM4A, DNA and amino acid sequence determination and analysis, quantitative PCR expression analysis and sequence comparisons
gene KDM4A, phylogenetic analysis, recombinant expression of N-terminally His-tagged KDM4A catalytic domain in Escherichia coli
gene KDM4A, real-time RT-PCR enzyme expression analysis
gene KDM4A, recombinant expression of EGFP-tagged full-length and truncated enzymes versions in U2-O2 cells
gene KDM4A, recombinant expression of FLAG-tagged JMJD2A in Spodoptera frugiperda Sf9 cells
gene KDM4A, recombinant expression of GFP-tagged enzyme-deficient mutant and of point mutants in fibroblasts
gene KDM4A, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
gene KDM4A, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3)
gene KDM4A, recombinant expression of His-tagged KDM4A residues 1-359 in Escherichia coli strain BL21 Codon-Plus-Ril from plasmid pNIC28-Bsa4
gene KDM4A, recombinant expression of N-terminally FLAG-tagged wild-type KDM4A or KDM4A H188A variant in U2OS cells and in HeLa cells, quantitative RT-PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged truncated KDM4A1-359 in Escherichia coli BL21(DE3)
gene KDM4A, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli
gene KDM4A, recombinant overexpression in U2O2 cells, recombinant expression of N-terminally FLAG-tagged KDM4A or KDM4A mutant H188A in HeLa cells, recombinant expression of N-terminally His6-tagged truncated KDM4A1-359 in Escherichia coli strain BL21(DE3)
gene KDM4A, short isoform of JMJD2A, DELTAN-JMJD2A, expression analysis, expression profiling of C2C12 cells, overview. DELTAN1-JMJD2A and DELTAN2-JMJD2A mRNAs are expressed at about 20% of the amount of the full-length mRNAs
gene KDM4A, transient recombinant overexpression in RPE cells
gene KDM4A/Jmjd2a, the loci encoding Jmjd2a and Jmjd2c are both located on chromosome 4 about 40 MB apart and are thus expected to co-segregate with a frequency related to the rate of meiotic recombination
gene KDM4B or Jmjd2b, real-time quantitative PCR expression analysis
gene KDM4B, phylogenetic analysis, recombinant expression of N-terminally His-tagged KDM4B catalytic domain in Escherichia coli
gene KDM4B, quantitative real-time PCR enzyme expression analysis
gene KDM4B, quantitative real-time RT-PCR expression analysis
gene KDM4B, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
gene KDM4B, recombinant isozyme expression in U2OS-TetON stable cell line that conditionally expresses the fusion protein EGFP-KDM4B
gene KDM4C, cloning of the Jmjd2c intronic DNA containing these Oct4-binding sites upstream of or downstream from a luciferase reporter to test for enhancer activity, robust enhancer activity is observed when the constructs is transfected into embryonic stem cells, real-time PCR expression analysis
gene KDM4C, phylogenetic analysis, recombinant expression of N-terminally His-tagged KDM4C catalytic domain in Escherichia coli
gene KDM4C, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
gene KDM4C, recombinant isozyme expression in U2OS-TetON stable cell line that conditionally expresses the fusion protein EGFP-KDM4C, recombinant expression of EGFP-tagged full-length and truncated, and mutant enzymes versions
gene KDM4C/Jmjd2c, the loci encoding Jmjd2a and Jmjd2c are both located on chromosome 4 about 40 MB apart and are thus expected to co-segregate with a frequency related to the rate of meiotic recombination
gene Kdm4d, expression analysis, recombinant expression in HeLa cells, di- and trimethylated H3K9 disappear from KDM4D-positive cells, whereas H3K9me1 were increased by KDM4D expression
gene KDM4D, functional recombinant expression of wild-type and mutant His6-tagged full-length enzyme in Escherichia coli strain BL21(DE3). To establish the U2OS-TetON stable cell line that conditionally expresses myc-KDM4D or EGFP-KDM4D-1H4R-HRK-FL, fragments including the myc-KDM4D or EGFP-KDM4D-1H4R-HRK-FL are subcloned into pTRE2-puro plasmid. The resulting pTRE2-puro-myc-KDM4D and pTRE2-puro-EGFP-KDM4D-1H4R-HRK-FL vectors are transfected into U2OS-Tet-ON cells. REcombinant expression of the His-tagged N-terminus, residues 1.350, of the enzyme, expressing the His-tagged C-terminus of KDM4D proves unsuccessful
gene KDM4D, phylogenetic analysis, recombinant expression of N-terminally His-tagged KDM4D catalytic domain in Escherichia coli
gene KDM4E, phylogenetic analysis, recombinant expression of N-terminally His-tagged KDM4E catalytic domain in Escherichia coli
gene kdmA or AN1060, phylogenetic analysis
gene KMD4A, recombinant expression of N-terminally His-tagged KDM4A construct (residues 1-359) in Escherichia coli, recombinant expression of N-terminally GST-tagged enzyme in Spodoptera frugiperda Sf9 cells via baculovirus transfection method
JMJD2A-C genes, DNA and amino acid sequence determination and analysis, expression of the GST-tagged isozymes JMJD2A, JMJD2B, and JMJD2C in Escherichia coli strain BL21(DE3)
-
JMJD2B quantitative real-time PCR expression analysis
JMJD2B quantitative RT-PCR expression analysis, overview
-
mouse Jmjd2b is transfected into JMJD2B-depleted MCF-7 cells, with stable transfection of JMJD2B-specific siRNA molecules
-
short isoform of JMJD2A expression analysis, overview
-