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H1120G/D1122N
a catalytically-inactive KDM3A mutant
H247A
complete loss of activity
H1560A/D1562A/H1689A
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catalytically inactive mutant, carries mutations in the conserved cofactor (Fe2+) binding site
D1012N
site-directed mutagenesis, reduced activity of the mutant compared to wild-type
D1012N
mutation identified in patients with atrichia with papular lesions, almost abolishes the demethylase activity
D1012N
the mutation abolishes the enzyme activity
F279S
site-directed mutagenesis
F279S
mutation identified in patients with X-linked mental retardation. abolishes catalytic activity
F279S
mutation identified in X-linked mental retardation patients, mutant is defective in enzymatic activity
H1120Y
inactive
H1120Y
loss of enzymatic activity
V1056M
site-directed mutagenesis, reduced activity of the mutant compared to wild-type
V1056M
mutation identified in patients with atrichia with papular lesions, almost abolishes the demethylase activity
V1056M
the mutation abolishes the enzyme activity
additional information
construction of two T-DNA insertion lines, jmj27-1 (SALK_092672) and jmj27-2 (SALK_131899), these mutants are null alleles. No visible phenotypic differences are observed in the mutant lines as compared with the wild-type Col-0, except that the mutants flowered earlier than the wild-type plants. Lesion mimic mutants (dll1, acd2, hrl1, lsd1) constitutively express several defense-related genes at high levels, and display enhanced resistance against a variety of pathogens
additional information
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homozygous mutant ibm1-4 (SALK_035608) is identified by PCR-based genotyping. The jmj24/ibm1 double mutant is generated by crossing jmj24-3 to ibm1-4. jmj24-2 is in the Wassilewskija background, whereas jmj24-3 and ibm1-4 are in the Columbia background
additional information
homozygous mutant ibm1-4 (SALK_035608) is identified by PCR-based genotyping. The jmj24/ibm1 double mutant is generated by crossing jmj24-3 to ibm1-4. jmj24-2 is in the Wassilewskija background, whereas jmj24-3 and ibm1-4 are in the Columbia background
additional information
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homozygous mutant ibm1-4 (SALK_035608) is identified by PCR-based genotyping. The jmj24/ibm1 double mutant is generated by crossing jmj24-3 to ibm1-4. jmj24-2 is in the Wassilewskija background, whereas jmj24-3 and ibm1-4 are in the Columbia background
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additional information
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LSD1 knockdown by siRNA inhibits 8-oxo-guanine production
additional information
small interfering RNA-mediated knockdown of LSD1
additional information
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ectopic expression of HR in HEK-293 cells leads to either upregulation or downregulation of the majority of the HR target genes, overview. HR overexpression can lead to H3K9 demethylation in the target gene promoters or transcription regulatory elements, TREs, enzyme overexpression leads to a partial demethylation of H3K9 within the TREs of Mxi1 and Caspase14
additional information
ectopic expression of HR in HEK-293 cells leads to either upregulation or downregulation of the majority of the HR target genes, overview. HR overexpression can lead to H3K9 demethylation in the target gene promoters or transcription regulatory elements, TREs, enzyme overexpression leads to a partial demethylation of H3K9 within the TREs of Mxi1 and Caspase14
additional information
enzyme knockdown by expression of specific sh-RNAs targeting PHF8 in HEK 293 T cells
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knockdown of JMJD1C in esophageal cancer cell lines, generation of Eca-109 and EC-18 EC cells that stably express JMJD1C shRNA
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knockdown of JMJD1C in esophageal cancer cell lines, generation of Eca-109 and EC-18 EC cells that stably express JMJD1C shRNA
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knockdown of KDM7A by siRNA transfection
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knockdown of KDM7A by siRNA transfection
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PHF8 enzyme knockout by PHF8 siRNA
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RNAi-mediated knockdown of KDM3A, Kdm3a knockdown 4T07 cells
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RNAi-mediated knockdown of KDM3A, Kdm3a knockdown 4T07 cells
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the enzyme is knocked out by expression of specific siRNA for LSD1 in chondrocytes
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construction of an enzyme mutant with a deletion of the NH2-terminal 184 amino acid residues (DELTA184 LSD1)
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down-regulation of the expression of JMJD1A using small interfering or short hairpin RNAs
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knockdown or knockout of PHF8 in 293T cells by RNAi or CRISPR-Cas9 system. The small guide RNAs (sgRNAs) target exon 8, which encodes amino acids 262-315 of the JmjC domain, and abolish PHF8 expression
additional information
knockdown or knockout of PHF8 in 293T cells by RNAi or CRISPR-Cas9 system. The small guide RNAs (sgRNAs) target exon 8, which encodes amino acids 262-315 of the JmjC domain, and abolish PHF8 expression
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LSD1 knockdown by transient siRNA transfection in HCT-116 cells
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MDA-MB231 cells are transiently transfected with three different siRNA directed against LSD1 causing significant knockdown of LSD1 in all samples on both mRNA and protein level. LSD1 knockdown leads to significant induction of the steady state transcript levels of interleukin 1alpha, 1beta, 6 and 8 genes. Interleukin 1beta protein levels are significantly enhanced in the supernatant of the cells after LSD1 knockdown
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small interference RNA-mediated depletion of both LSD1
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enzyme disruption mutant, and expression of a fusion protein consisting of the first 506 amino acids of the enzyme fused to a beta-galactosidase/neomycin cassette. The fusion protein lacks the catalytic domain. Presence of the active enzyme is essential for spermatogenesis. Mutant mice exhibit post-meiotic chromatin condensation defects. The enzyme binds directly to and controls the expression of transition nuclear protein 1 and protamine genes
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overexpression of wild-type Jmjd2c, but not of mutant H190A, increases the expression of Mdm2 oncogene dependent on its demethylase activity, which leads to the reduction of p53 tumor suppressor gene product in the cells
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generation of Kdm3a knockout mice
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construction of Jmjd1a knockout embryonic stem cells by RNAi assay, Jmjd1a depletion leads to embryonic stem cell differentiation, which is accompanied by a reduction in the expression of embryonic stem cell-specific genes and an induction of lineage marker genes. The same mutations that disrupt the in vitro Oct4/DNA interactions also abolish the enhancer activities. Knockdown of Jmjd1a does not appreciably affect Jmjd2c and vice versa
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RNAi knockdown of LSD1 expression leads to undetectable levels in about 90% of olfactory-placode-derived cell line (OP-6) cells in culture, modeling. Apparent systematic accumulation of H3K4me2 (and possibly H3K9me2) in LSD1-depleted cell populations, overview
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down-regulation of the expression of JMJD1A using small interfering or short hairpin RNAs
additional information
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down-regulation of the expression of JMJD1A using small interfering or short hairpin RNAs
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additional information
deletion of the swm1+ gene alters the expression patterns of several genes, overview
additional information
deletion of the swm1+ gene alters the expression patterns of several genes, overview
additional information
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deletion of the swm1+ gene alters the expression patterns of several genes, overview
additional information
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deletion of the swm1+ gene alters the expression patterns of several genes, overview
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additional information
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deletion of the swm1+ gene alters the expression patterns of several genes, overview
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