1.14.11.6: thymine dioxygenase

This is an abbreviated version!
For detailed information about thymine dioxygenase, go to the full flat file.

Reaction

Synonyms

5-hydroxy-methyluracil dioxygenase, 5-hydroxy-methyluracil oxygenase, EC 1.14.11.5, Fe2+/2-oxoglutarate dependent hydroxylase, J binding protein 1, J binding protein 2, J-binding protein 1, J-binding protein 2, JBP1, JBP2, More, T7H, thymidine hydroxylase, thymine 7-hydroxylase, thymine dioxygenase, thymine hydroxylase, thymine-7-hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

                1.14.11.6 thymine dioxygenase

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Results	in table
13	Activating Compound
109	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Crystallization (Commentary)
3	General Information
1	General Stability
28	Inhibitors
3	kcat/KM [mM/s]
4	Ki Value [mM]
33	KM Value [mM]
1	Localization
15	Metals/Ions
3	Molecular Weight [Da]
50	Natural Substrates/ Products (Substrates)
68	Organism
2	PDB ID
2	pH Optimum
42	Protein Variants
8	Purification (Commentary)
1	Reaction
5	Reaction Type
31	Reference
10	Source Tissue
3	Specific Activity [micromol/min/mg]
5	Storage Stability
98	Substrates and Products (Substrate)
4	Subunits
29	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
4	Temperature Stability [°C]
12	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.14.11.6 - thymine dioxygenase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-70°C, ammonium sulfate precipitate, several weeks, no activity loss

Rhodotorula glutinis

-

439141

0°C, ammonium sulfate precipitate, 5 h, quite stable

Rhodotorula glutinis

-

439141

20 mM potassium phosphate pH 7.5, 100 mM glycine and 0.1 mM EDTA, stable

Rhodotorula glutinis

-

439134

4°C, after dialysis against distilled water, one day or more

Neurospora crassa

-

439147

freezing at -79°C, storage at -39°C, 50 mM Tris-HCl buffer, pH 8.0, 1 mM glutathione, 1 mM ascorbate, 10 mM KCl, 8 days, 96% activity

Neurospora crassa

-

439146