Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.11.6: thymine dioxygenase

This is an abbreviated version!
For detailed information about thymine dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.6

Reaction

thymine
+
2-oxoglutarate
+
O2
=
5-Hydroxymethyluracil
+
succinate
+
CO2

Synonyms

5-hydroxy-methyluracil dioxygenase, 5-hydroxy-methyluracil oxygenase, EC 1.14.11.5, Fe2+/2-oxoglutarate dependent hydroxylase, J binding protein 1, J binding protein 2, J-binding protein 1, J-binding protein 2, JBP1, JBP2, More, T7H, thymidine hydroxylase, thymine 7-hydroxylase, thymine dioxygenase, thymine hydroxylase, thymine-7-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.6 thymine dioxygenase

Crystallization

Crystallization on EC 1.14.11.6 - thymine dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant full-length or truncated enzyme (unmodified or SeMet-labeled) in apo form, or complexed with 2-oxoglutarate, thymine, 5-hydroxymethyluracil, or 5-formyluracil, drop vapour diffusion method, 16°C, the reservoir solution consists of 0.2 M (NH4)2SO4, 0.1 M MES, pH 6.5, and 30% w/v PEGMME 5000 for the apoenzyme, for the 2-oxoglutarate complexed enzyme of 0.1 M bis-Tris, pH 5.5, and 25% w/v PEG 3,350, and 2-oxoglutarate in a 1:4 ratio protein/2-OG, or for enzyme in complex with other ligands in a 1:4:8 ratio, X-ray diffraction structure determination and analysis at 2.05-2.43 A resolution