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1.14.11.54: mRNA N1-methyladenine demethylase

This is an abbreviated version!
For detailed information about mRNA N1-methyladenine demethylase, go to the full flat file.

Word Map on EC 1.14.11.54

Reaction

N1-methyladenine in mRNA
+
2-oxoglutarate
 +
O2
=
adenine in mRNA
 +
formaldehyde
 +
succinate
 +
CO2

Synonyms

ABH3, AlkB, AlkB homolog 3, ALKBH3, alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, PCA-1, prostate cancer antigen-1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.54 mRNA N1-methyladenine demethylase

Engineering

Engineering on EC 1.14.11.54 - mRNA N1-methyladenine demethylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D135I
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
D135N
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
E136L
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
L177A
mutation at the active site, mutant lost its demethylation activity on m1A of tRNA in vitro and in vivo
R122A
mutant is defective in its DNA/RNA binding groove, mutant lost its demethylation activity on m1A of tRNA in vitro and in vivo