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1.14.11.53: mRNA N6-methyladenine demethylase

This is an abbreviated version!
For detailed information about mRNA N6-methyladenine demethylase, go to the full flat file.

Word Map on EC 1.14.11.53

Reaction

N6-methyladenine in mRNA
+
2-oxoglutarate
 +
O2
=
adenine in mRNA
 +
formaldehyde
 +
succinate
 +
CO2

Synonyms

AlkB homolog 5, ALKBH10B, ALKBH5, ALKBH5 demethylase, ALKBH9B, alkylation repair homolog protein 5, fat mass and obesity-associated enzyme, fat mass and obesity-associated protein, FTO, m6A mRNA demethylase, m6A RNA demethylase, m6A-RNA demethylase, N6-methyladenosine demethylase, RNA N6-methyladenine demethylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.53 mRNA N6-methyladenine demethylase

Cloned

Cloned on EC 1.14.11.53 - mRNA N6-methyladenine demethylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a plasmid with the vector backbone pNIC28-Bsa4 encoding a hexahistidine-tagged ALKBH566–292 construct is transformed into Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) Rosetta cells
-
expression in Escherichia coli BL21 Star (DE3)
expression in the BL21 (DE3) Escherichia coli strain
mutants of human Alkbh5 are amplified by PCR and subcloned into a modified pET-28a (Novagen) vector encoding a tobacco etch virus protease recognition site. The final clones are verified by DNA sequencing. All of the recombinant plasmids are transformed into Escherichia coli strain BL21(DE3)
the human ALKBH5 catalytic AlkB domain (residues 74–294) is subcloned into pET28a-MHL vector and expressed. The cloned vector is transformed into Escherichia coli BL21-V2R-pRARE2. Recombinant protein is produced at 16°C as an N-terminal His-tagged protein
Wild-type and various mutant enzymes cloned into pET-15b are expressed in Escherichia coli strain BL21