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1.14.11.48: xanthine dioxygenase

This is an abbreviated version!
For detailed information about xanthine dioxygenase, go to the full flat file.

Reaction

xanthine
+
2-oxoglutarate
+
O2
=
Urate
+
succinate
+
CO2

Synonyms

alpha-ketoglutarate-dependent xanthine hydroxylase, AO090001000152, TauD domain-containing protein, XanA, xanthine/alpha-ketoglutarate dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.48 xanthine dioxygenase

Purification

Purification on EC 1.14.11.48 - xanthine dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified from both the fungal mycelium and recombinant Escherichia coli cells
-
recombinant protein, the nickel affinity purified protein is subjected to ion-exchange chromatography once the N-terminal 6XHis-tag has been successfully removed, followed by size-exclusion purification
wild-type enzyme variants are purified from recombinant Escherichia coli cells