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1.14.11.48: xanthine dioxygenase

This is an abbreviated version!
For detailed information about xanthine dioxygenase, go to the full flat file.

Reaction

xanthine
+
2-oxoglutarate
+
O2
=
Urate
+
succinate
+
CO2

Synonyms

alpha-ketoglutarate-dependent xanthine hydroxylase, AO090001000152, TauD domain-containing protein, XanA, xanthine/alpha-ketoglutarate dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.48 xanthine dioxygenase

Engineering

Engineering on EC 1.14.11.48 - xanthine dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C357A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Mutant enzyme is resistant rto thiol-specific reagents that inactivated wild-type enzyme. Elevated ferroxidase activity in the absence of substrates
D138A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
D151A
inactive mutant enzyme
E137A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
H149A
inactive mutant enzyme
H340A
mutant enzyme exhibits 0.17% of the wild-type enzyme activity
K122A
2fold increase in the Kd of 2-oxoglutarate compared to wild-type enzyme
N358A
23fold decrease in kcat/Km compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
Q101A
elevated ferroxidase activity in the absence of substrates
Q356A
significant decrease in Ki(app) for 6,8-dihydroxypurine. Elevated ferroxidase activity in the absence of substrates