1.14.11.42: tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase

This is an abbreviated version!
For detailed information about tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase, go to the full flat file.

Reaction

7-(3-amino-3-carboxypropyl)wyosine37 in tRNAPhe
+
2-oxoglutarate
+
O2
=
7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe
+
succinate
+
CO2

Synonyms

tRNA wybutosine-synthesizing protein 5, tRNA yW-synthesizing enzyme 5, TYW5

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.42 tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase

Substrates Products

Substrates Products on EC 1.14.11.42 - tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-(3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + 2-oxoglutarate + O2
7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + succinate + CO2
show the reaction diagram
enzyme catalyzes the hydroxylation of the hypermodified nucleoside wybutoside in position 37 of tRNAPhe. Residues Y106 and L175 are required for 2-oxoglutarate binding. H160, N162, and H235 are residues in the HX(D/E)XnH motif that are required for Fe(II) binding
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?
additional information
?
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in the biogenesis of hydroxywybutosine, enzyme Tyw5p employs wybutosine residue 72 in tRNAPhe as a substrate by recognizing the N4-methyl group to hydroxylate the beta-carbon of the propyl group, followed by methylation and methoxycarbonylation of the side chain catalyzed by enzyme Tyw4 for completion
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