1.14.11.42: tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase

This is an abbreviated version!
For detailed information about tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase, go to the full flat file.

Reaction

7-(3-amino-3-carboxypropyl)wyosine37 in tRNAPhe
+
2-oxoglutarate
+
O2
=
7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe
+
succinate
+
CO2

Synonyms

tRNA wybutosine-synthesizing protein 5, tRNA yW-synthesizing enzyme 5, TYW5

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.42 tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase

General Information

General Information on EC 1.14.11.42 - tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase

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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
knock-down of enzyme gene by RNAi results in loss of hydroxywybutosine. Expression of the gene in yeast leads to formation of hydroxywybutosine. Enzyme Tyw5p employs wybutosine residue 72 in tRNAPhe as a substrate by recognizing the N4-methyl group to hydroxylate the beta-carbon of the propyl group, followed by methylation and methoxycarbonylation of the side chain catalyzed by enzyme Tyw4 for completion