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1.14.11.33: DNA oxidative demethylase

This is an abbreviated version!
For detailed information about DNA oxidative demethylase, go to the full flat file.

Word Map on EC 1.14.11.33

Reaction

DNA-base-CH3
+
2-oxoglutarate
 +
O2
=
DNA-base
 +
formaldehyde
 +
succinate
 +
CO2

Synonyms

1-methyladenine-DNA dioxygenase, ABH1, ABH2, ABH3, AlkB, AlkB homolog 1, ALKBH2, ALKBH3, ALKBH8, alkylated DNA repair protein, alpha-ketoglutarate-dependent dioxygenase ABH1, FTO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.33 DNA oxidative demethylase

Engineering

Engineering on EC 1.14.11.33 - DNA oxidative demethylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D61A
-
the mutant is unable to rescue methylated RNA phage MS2 and is unable to convert 2-oxoglutarate to succinate
H59A
-
the mutant is unable to rescue methylated RNA phage MS2 and is unable to convert 2-oxoglutarate to succinate
D133C
-
the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme
D135A
the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0
D135I
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
D135N
D135S
the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0
E136L
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
H131C
-
the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme
H187C
-
the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme
C118A
-
the mutant shows wild type activity
C118A/C129A
-
the mutant shows wild type activity
C129A
-
the mutant shows wild type activity
C129A/H134A
-
the mutant shows 65% of wild type activity
C304A
-
the mutant shows wild type activity
C304A/C371A
-
the mutant shows wild type activity
C371A
-
the mutant shows wild type activity
C371A/H372A
-
the mutant shows wild type activity
D173A
-
site-directed mutagenesis, the mutant maintains nucleolar localization, but is not able to enhance transcription from the rDNA luciferase reporter. eÉctopic expression of wild-type ABH2 leads to a 2.5fold increase of pre-rRNA, whereas ectopic expression of D173A mutant only leads to a marginal increase of pre-rRNA
H113A
-
the mutant shows wild type activity
H113A/C118A
-
the mutant shows 83% of wild type activity
H113A/C118A/C129A/H134A
-
the mutant shows 69% of wild type activity
H113A/C129A
-
the mutant shows wild type activity
H131C
-
the mutant slightly prefers single-stranded DNA substrates over any double-stranded DNA
H134A
-
the mutant shows wild type activity
H171C
-
the ABH2 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins and cross-links efficiently with the single-stranded DNA-1 and DNA3-6
H191C
-
the ABH3 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins and cross-links to DNA-1 much more efficiently than the wild type protein after a 20 h incubation
H231C
-
the ABH1 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins, no cross-link between the mutant protein and the single-stranded and double-stranded DNA probes is observed
H303A
-
the mutant shows wild type activity
H303A/C304A
-
the mutant shows wild type activity
H372A
-
the mutant shows wild type activity