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1.14.11.17: taurine dioxygenase

This is an abbreviated version!
For detailed information about taurine dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.17

Reaction

taurine
+
2-oxoglutarate
+
O2
=
sulfite
+
aminoacetaldehyde
+
succinate
+
CO2

Synonyms

2-aminoethanesulfonate dioxygenase, 2-aminoethanesulfonic acid/alpha-ketoglutarate dioxygenase, alpha-ketoglutarate-dependent dioxygenase, alpha-ketoglutarate-dependent taurine dioxygenase, alpha-ketoglutarate/taurine dioxygenase, AtsK, Fe(II)/2-oxoglutarate-dependent taurine dioxygenase, Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenase, oxygenative alkylsulfatase, SSI3, TauD, TauD-{FeNO}7, taurine (2-aminoethanesulfonate)/2-oxoglutarate dioxygenase, taurine alpha ketoglutarate dioxygenase, taurine alpha-ketoglutarate dioxygenase, taurine dioxygenase, taurine hydroxylase, taurine-alpha-ketoglutarate dioxygenase, taurine/2-oxoglutarate dioxygenase, taurine/alpha-ketoglutarate dioxygenase, taurine/alpha-ketoglutarate-dependent dioxygenase, taurine/alphaKG dioxygenase, taurine/alphaKGD, taurine: alpha-ketoglutarate dioxygenase, taurine:2OG dioxygenase, taurine:alpha-ketoglutarate dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.17 taurine dioxygenase

Purification

Purification on EC 1.14.11.17 - taurine dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose column chromatography and HP phenyl-Sepharose column chromatography
-
dialysis against 25 mM Tris buffer at pH 8.0
-
recombinant His-tagged TauD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant protein using His-tag
Resource-Q anion-exchange column chromatography and Superdex 200 gel filtration
-
two-step purification from overexpressing Escherichia coli to apparent homogeneity
-