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1.13.12.7: firefly luciferase

This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.

Word Map on EC 1.13.12.7

Reaction

D-firefly luciferin
+
O2
+
ATP
=
firefly oxyluciferin
+
CO2
+
AMP
+
diphosphate
+
hnu

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.7 firefly luciferase

pH Range

pH Range on EC 1.13.12.7 - firefly luciferase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 10.5
pH activity range, recombinant circular enzyme
4 - 10
pH activity range, recombinant wild-type enzyme
6 - 7.8
bioluminescence spectra independent from pH, for mutants Y35N, Y35H and F16L/A40S
6.5 - 8.5
-
pH 6.5: about 45% of activity maximum, pH 8.5: about 35% of activity maximum
6.5 - 9.6
-
-
7 - 9
-
pH 7.0: about 80% of maximal activity, pH 9.0: about 70% of maximal activity, isoenzyme AL1
7.5 - 8
7.5 - 8.5
7.5 - 9.5
-
pH 7.5: about 50% of maximal activity, pH 9.5: about 50% of maximal activity, isoenzyme AL2
8.2 - 9.1
-
-
additional information
-
buffers with pKa from 7.2 to 8.4 tested and no relationship with activity found