1.13.12.7: firefly luciferase
This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.
Word Map on EC 1.13.12.7
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1.13.12.7
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bioluminescence
-
luminescence
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chemiluminescence
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emit
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renilla
-
noninvasive
-
luciferases
-
cypridina
-
photon
-
lentiviral
-
adenovirus
-
click
-
luciferase-expressing
-
herpes
-
engraft
-
nude
-
co-transfected
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cytomegalovirus
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camera
-
electroporation
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simplex
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hsp70
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emitter
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replication-deficient
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excited-state
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promoter-driven
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charge-coupled
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nonviral
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nanoluc
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non-invasively
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gaussia
-
luminol
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intramyocardial
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coelenterazine
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bicistronic
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yellow-green
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molecular biology
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light-emitting
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cap-independent
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aequorin
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biotechnology
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red-shifted
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dnaj
-
luminometer
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grpe
-
polyethylenimine
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bioimaging
-
luciferase-based
-
medicine
-
photoproteins
-
glow
-
analysis
-
multicolor
-
brighter
- 1.13.12.7
-
bioluminescence
-
luminescence
-
chemiluminescence
-
emit
- renilla
-
noninvasive
- luciferases
- cypridina
-
photon
-
lentiviral
- adenovirus
-
click
-
luciferase-expressing
-
herpes
-
engraft
-
nude
-
co-transfected
- cytomegalovirus
-
camera
-
electroporation
- simplex
- hsp70
-
emitter
-
replication-deficient
-
excited-state
-
promoter-driven
-
charge-coupled
-
nonviral
- nanoluc
-
non-invasively
- gaussia
- luminol
-
intramyocardial
- coelenterazine
-
bicistronic
-
yellow-green
- molecular biology
-
light-emitting
-
cap-independent
- aequorin
- biotechnology
-
red-shifted
- dnaj
-
luminometer
- grpe
- polyethylenimine
-
bioimaging
-
luciferase-based
- medicine
-
photoproteins
-
glow
- analysis
-
multicolor
-
brighter
Reaction
Synonyms
AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT
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Renatured
Renatured on EC 1.13.12.7 - firefly luciferase
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in the presence of the DnaKJE chaperone system thermally inactivated monomeric bacterial luciferase refolds. Monomeric bacterial luciferase thermally inactivated in the presence of ATPindependent trigger factor is not able to refold
renaturation is initiated by diluting the denaturant in 50 mM HEPES-KOH, pH 7.5, 10 mM Mg(OAc)2, 70 mM KOAc, 50 mM imidazole, and 1 mM dithiothreitol without urea, refolding of firefly luciferase from a denatured state is a slow process, its rate and productivity depend on molecular chaperones of the Hsp70 family, Hsp70-dependent refolding restores 55% of the initial enzymatic activity, immobilization leads to a higher refolding yield owing to the prevention of intermolecular aggregation