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1.13.12.7: firefly luciferase

This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.

Word Map on EC 1.13.12.7

Reaction

D-firefly luciferin
+
O2
 +
ATP
=
firefly oxyluciferin
 +
CO2
 +
AMP
 +
diphosphate
 +
hnu

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.7 firefly luciferase

Activating Compound

Activating Compound on EC 1.13.12.7 - firefly luciferase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-synuclein
-
dose-dependent enhancement of oxyluciferin formation, maximum rate of bioluminescence independent upon ATP concentration
-
diphosphate
stimulates light production under some conditions
Luciferin
-
preparations supplemented with extra luciferin provide maximum light emission
molecular oxygen
Polyethyleneglycol
-
stimulates activity by promoting the dissociation of inhibitory product from the enzyme
Polyvinylpyrrolidone
-
stimulates activity by promoting the dissociation of inhibitory product from the enzyme
Triphosphate
stimulates light production under some conditions
Triton X-100
-
stimulates activity by promoting the dissociation of inhibitory product from the enzyme
[1,1,3,3-tetramethylguanidine][acetate]
-
enzyme shows increased activity and increased thermal stability
[1,1,3,3-tetramethylguanidine][lactate]
-
increase in concentration of [1,1,3,3-tetramethylguanidine][Lac] up to 0.25 M increases enzyme activity, and at concentrations more than 0.25 M enzyme activity is decreased. Optimum temperature and thermal stability studies show more stability of luciferase only in the presence of [1,1,3,3-tetramethylguanidine][Lac]
[1,1,3,3-tetramethylguanidine][propionate]
-
in the presence of [1,1,3,3-tetramethylguanidine][propionate], enzyme activities are decreased with an increase in ionic liquid concentration
[1,1,3,3-tetramethylguanidine][trichloroacetate]
-
enzyme shows decreased activity
[1,1,3,3-tetramethylguanidine][trifluoroacetate]
-
enzyme shows unchanged activity
additional information
-