1.13.12.6: Cypridina-luciferin 2-monooxygenase

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For detailed information about Cypridina-luciferin 2-monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.6

1.13.12.6

bioluminescence

luminescence

hilgendorfii

luciferases

ostracod

firefly

analysis

gaussia

diagnostics

molecular biology

biotechnology

Reaction

Synonyms

Apogon luciferase, Apogon luciferase 1, CLase, CLuc, Cypridina luciferase, Cypridina luciferin 2-monooxygenase, Cypridina noctiluca luciferase, Cypridina-type luciferase, Fbp, FBP-IgG, luciferase (Cypridina luciferin), PGE2-luciferase, Vargula luciferase

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

1.13.12.6 Cypridina-luciferin 2-monooxygenase

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Results	in table
1	Activating Compound
5	AA Sequence
15	Application
1	CAS Registry Number
11	Cloned(Commentary)
4	General Information
2	General Stability
10	Inhibitors
10	KM Value [mM]
5	Localization
3	Metals/Ions
8	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
33	Organism
5	pH Optimum
1	pH Range
1	pI Value
3	Posttranslational Modification
16	Protein Variants
10	Purification (Commentary)
2	Reaction
3	Reaction Type
33	Reference
6	Source Tissue
1	Specific Activity [micromol/min/mg]
5	Storage Stability
31	Substrates and Products (Substrate)
6	Subunits
35	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Inhibitors

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Inhibitors on EC 1.13.12.6 - Cypridina-luciferin 2-monooxygenase

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alpha,alpha'-dipyridyl

slight inhibition

chlorpromazine

which binds to human plasma alpha 1-acid glycoprotein, shows an inhibitory effect on the luminescence of Cypridina luciferin, both in the presence of human plasma alpha 1-acid glycoprotein and a recombinant Cypridina luciferase

764911

Cypndina gamma-globulin

Vargula hilgendorfii

Cypridina luciferase is completely inhibited by a rabbit antibody to Cypridina luciferase

dithiothreitol

Sodium azide

slight inhibition

sodiumdodecylsulfate

approx. 1 mM, complete inactivation

439325

Urea

Cypridina sp.

at 5.0 M urea, the half-time of the enzyme is 45 min, at 6.0 M urea 8 min, and at 7.5 M urea, the enzyme is inactivated within 1 min. r´Reversible, non-competitive inhibition of the luciferase activity occurs at concentrations up to 1.5 M

744465

additional information

2 entries