1.13.12.22: deoxynogalonate monooxygenase

This is an abbreviated version!
For detailed information about deoxynogalonate monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.22

Reaction

deoxynogalonate
+
O2
=
nogalonate
+
H2O

Synonyms

NMO, nogalamycin monooxygenase, SnoaB

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.22 deoxynogalonate monooxygenase

Engineering

Engineering on EC 1.13.12.22 - deoxynogalonate monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H49A
-
kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 13% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag
R90E
-
kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 17% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag
W67F
-
kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 0.5% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag