1.13.12.16: nitronate monooxygenase
This is an abbreviated version!
For detailed information about nitronate monooxygenase, go to the full flat file.
Word Map on EC 1.13.12.16
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1.13.12.16
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nitroalkanes
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flavin
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nitroethane
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neurospora
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crassa
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fmn
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hansenula
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mrakii
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flavosemiquinone
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1-nitropropane
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denitrification
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3-nitropropionic
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ansochromogenes
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fmn-dependent
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saturnus
- 1.13.12.16
- nitroalkanes
- flavin
- nitroethane
- neurospora
- crassa
- fmn
- hansenula
- mrakii
-
flavosemiquinone
- 1-nitropropane
-
denitrification
-
3-nitropropionic
- ansochromogenes
-
fmn-dependent
- saturnus
Reaction
Synonyms
2-nitropropane dioxygenase, EC 1.13.11.32, NAO, ncd2, nitroalkane oxidase, nitroalkane-oxidizing enzyme, nitronate monooxygenase, nitropropane dioxygenase, NMO, NMO-Nc, NMO-Ws, nmoA, Npd1, Npd2, Npd3, Npd4, Npd5, Npd6, oxidase, nitroalkane, oxygenase, 2-nitropropane di-, P3N monooxygenase, Pa-NMO, PA4202, protein PA4202, Rv1894c, Sa-NAO
ECTree
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Cloned
Cloned on EC 1.13.12.16 - nitronate monooxygenase
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expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
gene ncd-2, expression in Escherichia coli strain BL21(DE3), subcloning in strain XL-1 Blue
gene ncd-2, expression in Escherichia coli strain BL21(DE3), subcloning in strain XL-1 Blue; expressed in Escherichia coli BL21(DE3) cells
gene PA4202 or nmoA, monocistronic, low expression level gene, has an intergenic region of 97 nucleotides with neighbouring gene PA4201 (ddlA) encoding D-alanine alanine ligase, and an intergenic region of 107 nucleotides with gene PA4203 encoding a LysR regulator, PA4203 (nmoR) represses its own transcription and the expression of PA4202 (nmoA), presence of a single NmoR binding site between nmoA and nmoR, and interaction of NmoR with the intergenic nmoA-nmoR control region. In silico analysis of the ddlA-nmoA-nmoR-ppgL locus in Pseudomonas aeruginosa genomes, transcriptome analysis
gene pa4202, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta(DE3)pLysS