1.13.12.13: Oplophorus-luciferin 2-monooxygenase

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For detailed information about Oplophorus-luciferin 2-monooxygenase, go to the full flat file.

Reaction

Synonyms

19kOLase, CXXC-Oluc, imidazopyrazinone-type luciferase, KAZ, luciferase, nanoKAZ, NanoLuc, Oluc-19, Oplophorus luciferase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.13 Oplophorus-luciferin 2-monooxygenase

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Results	in table
1	AA Sequence
3	Application
8	Cloned(Commentary)
1	Crystallization (Commentary)
4	General Information
21	Inhibitors
18	Ki Value [mM]
4	KM Value [mM]
4	Localization
4	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
14	Organism
15	PDB ID
4	pH Optimum
29	Protein Variants
6	Purification (Commentary)
1	Reaction
14	Reference
1	Renatured (Commentary)
1	Source Tissue
4	Specific Activity [micromol/min/mg]
44	Substrates and Products (Substrate)
5	Subunits
18	Synonyms
1	Systematic Name
2	Temperature Stability [°C]

General Information

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General Information on EC 1.13.12.13 - Oplophorus-luciferin 2-monooxygenase

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

evolution

Oplophorus gracilirostris

Q9GV45; Q9GV46

the enzyme is a member of the coelenterazine-utilizing luciferases such as Renilla luciferase and Gaussia luciferase

744231

malfunction

Oplophorus gracilirostris

Q9GV45; Q9GV46

recombinant mutant nanoKAZ lacks the amino-terminal signal peptide is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway

744223

physiological function

Oplophorus gracilirostris

Q9GV45; Q9GV46

the secreted luciferase of the deep-sea shrimp Oplophorus gracilirostris catalyzes the oxidation of coelenterazine to emit blue light (lambda_max = 460 nm)

744220

additional information

Oplophorus gracilirostris

Q9GV45; Q9GV46

the coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the beta-barrel structure of the catalytic subunit KAZ

744231