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1.13.11.B6: linoleate 9/13-lipoxygenase

This is an abbreviated version!
For detailed information about linoleate 9/13-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.B6

Reaction

2 linoleate + 2 O2 =

(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
+
(9Z,11E)-13-hydroperoxy-9,11-octadecadienoate

Synonyms

13/9 lipoxygenase, 13/9-LOX, 9-/13-LOXN2, 9-lipoxygenase, 9-LOX, 9/13-lipoxygenase, 9/13-LOX, Ca-LOC-1, dual positional specific LOX, linoleate 9 S-lipoxygenase, linoleate oxygen oxidoreductase, linolenate 9R-dioxygenase, lipoxygenase, LOX, lox1-St-2, LOX1-Zm1, LOX1:Md:1a, LOX1a, LOX2:Md:2a, LOX2a, manganese 9S-lipoxygenase, MoLOX1, OsLOX1, PnLOX1, S-LOX

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.B6 linoleate 9/13-lipoxygenase

Engineering

Engineering on EC 1.13.11.B6 - linoleate 9/13-lipoxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G567A
I566F
site-directed mutagenesis, inactive mutant
I578L
site-directed mutagenesis, mutant substrate specificity compared to the wild-type enzyme, chiral analysis
L572I
site-directed mutagenesis, inactive mutant
R268A
site-directed mutagenesis, mutant substrate specificity compared to the wild-type enzyme, chiral analysis
V582F
site-directed mutagenesis, mutant substrate specificity compared to the wild-type enzyme, chiral analysis
Q599F
the wild-type enzyme is a linoleate 13-LOX above pH 7.5, below this value it functions as a linoleate 9-LOX. The mutant loses its 13-LOX activity at higher pH-values. At pH-values above 7.5 a rather unspecific product pattern (1:1 ratio of 13-hydroperoxy-(10E,12Z)-octadecadienoate and 9-hydroperoxy-(10E,12Z)-octadecadienoate) is observed with the mutant enzyme. The unspecific oxygenation reaction is also indicated by the R/S-ratio (4:6) of the major reaction products
Q599H
the mutation converts the enzyme to a linoleate 13-LOX lacking any pH sensitivity. The pH optimum remains unaffected, and the major product isomers are in the S-configuration
L350M
F580A
F580V
-
possesses activity profile that is similar to wild-type
K578/T579RS
-
possesses activity profile that is similar to wild-type
L569V
-
kcat/KM for linoleate hydroperoxidation is reduced by 38%
T579F
-
kcat/KM for linoleate hydroperoxidation is reduced by 89%
T579S
-
possesses activity profile that is similar to wild-type
V570I
-
possesses activity profile that is similar to wild-type
W523A
-
kcat/KM for linoleate hydroperoxidation is reduced by 98%
I437L
KM-value for linoleate is 114% of wild-type value, kcat/Km for linoleate is 83% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.7 (compared to 0.8 in reaction with wild-type enzyme)
V570I
KM-value for linoleate is 61% of wild-type value, kcat/Km for linoleate is 367% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.6 (compared to 0.8 in reaction with wild-type enzyme)
V580F
KM-value for linoleate is 384% of wild-type value, kcat/Km for linoleate is 4% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.9 (compared to 0.8 in reaction with wild-type enzyme)
A562G
additional information