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1.13.11.88: isoeugenol monooxygenase

This is an abbreviated version!
For detailed information about isoeugenol monooxygenase, go to the full flat file.

Word Map on EC 1.13.11.88

Reaction

isoeugenol
+
O2
=
Vanillin
 +
acetaldehyde

Synonyms

iem, Iem720, ISO, NCED

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.88 isoeugenol monooxygenase

Inhibitors

Inhibitors on EC 1.13.11.88 - isoeugenol monooxygenase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
66.54% residual activity in the presence of 5 mM 1,10-phenanthroline
8-hydroxyquinoline
1 mM, 90% loss of activity
Ag+
1 mM, 73% loss of activity
Ca2+
-
64.6% residual activity at 10 mM
EDTA
64.77% residual activity in the presence of 5 mM EDTA
ethyl acetate
in the presence 10% (v/v) ethyl acetate, the enzyme shows 19.35% residual activity with isoeugenol and 35.06% residual activity with 4-vinylguaiacol as substrate
Fe2+
70.55% residual activity in the presence of 5 mM Fe2+
Hg2+
1 mM, 87% loss of activity
K+
-
about 90 % residual activity at 10 mM
Mg2+
-
about 90 % residual activity at 10 mM
Mn2+
-
69.4% residual activity at 10 mM
n-Butyl alcohol
in the presence of 10% (v/v) n-butyl alcohol, the enzyme shows 38.19% residual activity with isoeugenol and 21.44% residual activity with 4-vinylguaiacol as substrate
n-caprylic alcohol
in the presence of 10% (v/v) n-caprylic acid, the enzyme shows 74.73% residual activity with isoeugenol as substrate
-
n-octane
in the presence of 10% (v/v) n-octane, the enzyme shows 55.34% residual activity with isoeugenol and 70.45% residual activity with 4-vinylguaiacol as substrate
p-chloromercuribenzoic acid
1 mM, 44% loss of activity
petroleum ether
in the presence of 10% (v/v) petroleum ether, the enzyme shows 46.05% residual activity with isoeugenol as substrate
-
phenylhydrazine
1 mM, 95% loss of activity
R-cycloserine
1 mM, 48% loss of activity
Toluene
in the presence of 10% (v/v) toluene, the enzyme shows 39.56% residual activity with isoeugenol and 29.36% residual activity with 4-vinylguaiacol as substrate
Triton X-100
66.54% residual activity in the presence of 5 mM Triton X-100
Vanillin
-
there is an incremental decrease in enzyme activity with the increasing vanillin concentration
additional information
-