1.13.11.88: isoeugenol monooxygenase
This is an abbreviated version!
For detailed information about isoeugenol monooxygenase, go to the full flat file.
Word Map on EC 1.13.11.88
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1.13.11.88
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vanillin
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nitroreducens
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putida
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phenylpropanoids
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fragrance
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synthesis
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bl21de3
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trans-anethole
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acetaldehyde
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pet21a
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undesired
- 1.13.11.88
- vanillin
- nitroreducens
- putida
-
phenylpropanoids
-
fragrance
- synthesis
-
bl21de3
- trans-anethole
- acetaldehyde
-
pet21a
-
undesired
Reaction
Synonyms
iem, Iem720, ISO, NCED
ECTree
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Application
Application on EC 1.13.11.88 - isoeugenol monooxygenase
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synthesis
Escherichia coli cells expressing isoeugenol monooxygenase produce 28.3 g vanillin/l from 230 mM isoeugenol, with a molar conversion yield of 81% at 20°C after 6 h. No accumulation of undesired by-products, such as vanillic acid or acetaldehyde, is observed
synthesis
the vanillin producing activity is induced by presence of isoeugenol. Under the optimized reaction conditions, Pseudomonas putida cells produce 16.1 g/l vanillin from 150 mM isoeugenol, with a molar conversion yield of 71% at 20 °C after a 24-h incubation in the presence of 10% (v/v) dimethyl sulfoxide
synthesis
upon expression of mutant F281Q in Escherichia coli and employing sol-gel chitosan membrane for removing the produced vanillin from the biotransformation system, under the optimal conditions (pH 8.5, 30°C, 180 rpm, 0.5 g wet cells, 0.1 g chitosan membrane), vanillin concentrations reach 4.5 g/l after about 40 h