1.13.11.86: 5-aminosalicylate 1,2-dioxygenase

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For detailed information about 5-aminosalicylate 1,2-dioxygenase, go to the full flat file.

5AS 1,2-dioxygenase, mabB

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Results	in table
1	AA Sequence
1	Cloned(Commentary)
1	Expression
3	General Information
3	Inhibitors
2	KM Value [mM]
2	Metals/Ions
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
2	Organism
1	pH Optimum
1	pH Range
1	pH Stability
4	Protein Variants
2	Purification (Commentary)
1	Reaction
2	Reference
1	Storage Stability
7	Substrates and Products (Substrate)
1	Subunits
3	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]

Engineering on EC 1.13.11.86 - 5-aminosalicylate 1,2-dioxygenase

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G93A

the mutant enzyme shows the ability to oxidize salicylate, the wild-type enzyme shows no activity with salicylate

H107A

mutant enzyme shows no enzymatic activity

H109A

mutant enzyme shows no enzymatic activity

H150A

mutant enzyme shows no enzymatic activity