1.13.11.79: aerobic 5,6-dimethylbenzimidazole synthase
This is an abbreviated version!
For detailed information about aerobic 5,6-dimethylbenzimidazole synthase, go to the full flat file.
Reaction
Synonyms
BluB, bluB/cobT2, DMB-synthase, EC 1.14.99.40, flavin destructase, flavin destructase enzyme
ECTree
Advanced search results
General Information
General Information on EC 1.13.11.79 - aerobic 5,6-dimethylbenzimidazole synthase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
metabolism
physiological function
additional information
BluB/CobT2 is the crucial enzyme in the B12 biosynthetic pathway of Propionibacterium freudenreichii
metabolism
-
the enzyme gene is required for aerobic 5,6-dimethylbenzimidazole synthesis for cobalamin production
metabolism
-
BluB/CobT2 is the crucial enzyme in the B12 biosynthetic pathway of Propionibacterium freudenreichii
-
-
a Tn5 insertion mutant is unable to grow in minimal media and fails to establish a symbiosis with alfalfa. Effect can be rescued by addition of vitamin B12 or of 5,6-dimethylbenzimidazole. Mutant does not produce cobalamin unless 5,6-dimethylbenzimidazole is supplied
physiological function
enzyme deletion mutant is unable to convert Mg-protoporphyrin IX monomethyl ester into protochlorophyllide, mutant starin grows poorly under anoxic photoheterotrophic conditions
physiological function
the enzyme is involved in production of vitamin B12, a prerequisite for attempts to naturally fortify foods with B12 by microbial fermentation. Active vitamin B12 is distinguished from the pseudovitamin by the presence of 5,6-dimethylbenzimidazole (DMBI) as the lower ligand. Fusion enzyme BluB/CobT2 is efficient in metabolite channeling, and the enzymes' inability to react with adenine, a lower ligand present in the pseudovitamin, reveals a mechanism favoring the production of the active form of the vitamin, requirement of oxygen for DMBI synthesis, since the organism does not synthesize cobalamin. BluB is responsible for the formation of DMBI from FMNH2 in the presence of oxygen, BluB/CobT2 activates DMBI into alpha-RP in the presence of NaMN
physiological function
-
the enzyme is involved in production of vitamin B12, a prerequisite for attempts to naturally fortify foods with B12 by microbial fermentation. Active vitamin B12 is distinguished from the pseudovitamin by the presence of 5,6-dimethylbenzimidazole (DMBI) as the lower ligand. Fusion enzyme BluB/CobT2 is efficient in metabolite channeling, and the enzymes' inability to react with adenine, a lower ligand present in the pseudovitamin, reveals a mechanism favoring the production of the active form of the vitamin, requirement of oxygen for DMBI synthesis, since the organism does not synthesize cobalamin. BluB is responsible for the formation of DMBI from FMNH2 in the presence of oxygen, BluB/CobT2 activates DMBI into alpha-RP in the presence of NaMN
-
identification of catalytic residues involved in the reaction, mutational analysis, overview. The enzyme shows interactions with the phosphate group and ribityl tail of FMN
additional information
-
identification of catalytic residues involved in the reaction, mutational analysis, overview. The enzyme shows interactions with the phosphate group and ribityl tail of FMN