1.13.11.78: 2-amino-1-hydroxyethylphosphonate dioxygenase (glycine-forming)
This is an abbreviated version!
For detailed information about 2-amino-1-hydroxyethylphosphonate dioxygenase (glycine-forming), go to the full flat file.
Reaction
Synonyms
(R)-1-hydroxy-2-aminoethylphosphonate oxygenase, ALOHA_HF130_AEPn_1_06c, diiron oxygenase, phnZ
ECTree
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Engineering
Engineering on EC 1.13.11.78 - 2-amino-1-hydroxyethylphosphonate dioxygenase (glycine-forming)
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E27A
-
active site variant with severely reduced catalytic efficiency compared to the wild type enzyme
H62A
-
active site variant with strongly reduced catalytic efficiency compared to the wild type enzyme
Y24F
-
active site variant with strongly reduced catalytic efficiency compared to the wild type enzyme
D161A
site-directed mutagenesis, the mutant is inactive
D59A
site-directed mutagenesis, the mutant is unstable
H104A
site-directed mutagenesis, the mutant is inactive
H34A
site-directed mutagenesis, the mutant is inactive
H58A
site-directed mutagenesis, the mutant is inactive
H80A
site-directed mutagenesis, the mutant shows 10fold reduced activity compared to the wild-type enzyme, reduced Fe-enzyme molar ratios exist in the variants H80A