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1.13.11.77: oleate 10S-lipoxygenase

This is an abbreviated version!
For detailed information about oleate 10S-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.77

Reaction

alpha-linolenate
+
O2
=
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate

Synonyms

(10S)-dioxygenase, (10S)-DOX, 10-dioxygenase, 10S-dioxygenase, 10S-DOX, 10S-DOX-like lipoxygenase, heme dioxygenase, linoleate 10S-dioxygenase, linoleate 10S-DOX, linoleate heme 10S-dioxygenase, LOX, Np-diox, Npun_R5469, oleate heme 10S-dioxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.77 oleate 10S-lipoxygenase

Engineering

Engineering on EC 1.13.11.77 - oleate 10S-lipoxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
optimization of conditions for 10S-hydroxy-8(E)-octadecenoic acid production from oleic acid by recombinant Escherichia coli cells expressing Nostoc punctiforme 10S-dioxygenase with the aid of a chaperone plasmid. Optimal conditions are pH 9.0, 35°C, 15 % v/v dimethyl sulfoxide, 40 g cells/l, and 10 g oleic acid/l. The activity of recombinant cells expressing 10S-dioxygenase is increased by 200% with the aid of chaperone Gro7. Production of 10S-hydroxy-8(E)-octadecenoic acid, 10S-hydroxy-8,12(E,Z)-octadecadienoic acid, and 10S-hydroxy-8,12,15(E,Z,Z)-octadecatrienoic acid from unsaturated fatty acids including oleic acid, linoleic acid, and a-linolenic acid, respectively. The soluble 10S-dioxygenase content in the crude extract of recombinant cells co-expressing the 10S-dioxygenase gene with pGro7 is higher than that of recombinant cells co-expressing pKJE7 or pG-KJE8. Production of 360 mg/g/h 10S-hydroxy-8(E)-octadecenoic acid from oleic acid