1.13.11.77: oleate 10S-lipoxygenase
This is an abbreviated version!
For detailed information about oleate 10S-lipoxygenase, go to the full flat file.
Word Map on EC 1.13.11.77
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1.13.11.77
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indoleamine
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oleic
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nostoc
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aeruginosa
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hydroperoxide
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l-tryptophan
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nicotinamide
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cyclooxygenases
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punctiforme
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indole
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2,3-dioxygenases
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nad+
- 1.13.11.77
- indoleamine
-
oleic
- nostoc
- aeruginosa
- hydroperoxide
- l-tryptophan
- nicotinamide
-
cyclooxygenases
- punctiforme
- indole
-
2,3-dioxygenases
- nad+
Reaction
Synonyms
(10S)-dioxygenase, (10S)-DOX, 10-dioxygenase, 10S-dioxygenase, 10S-DOX, 10S-DOX-like lipoxygenase, heme dioxygenase, linoleate 10S-dioxygenase, linoleate 10S-DOX, linoleate heme 10S-dioxygenase, LOX, Np-diox, Npun_R5469, oleate heme 10S-dioxygenase
ECTree
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Engineering
Engineering on EC 1.13.11.77 - oleate 10S-lipoxygenase
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additional information
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optimization of conditions for 10S-hydroxy-8(E)-octadecenoic acid production from oleic acid by recombinant Escherichia coli cells expressing Nostoc punctiforme 10S-dioxygenase with the aid of a chaperone plasmid. Optimal conditions are pH 9.0, 35°C, 15 % v/v dimethyl sulfoxide, 40 g cells/l, and 10 g oleic acid/l. The activity of recombinant cells expressing 10S-dioxygenase is increased by 200% with the aid of chaperone Gro7. Production of 10S-hydroxy-8(E)-octadecenoic acid, 10S-hydroxy-8,12(E,Z)-octadecadienoic acid, and 10S-hydroxy-8,12,15(E,Z,Z)-octadecatrienoic acid from unsaturated fatty acids including oleic acid, linoleic acid, and a-linolenic acid, respectively. The soluble 10S-dioxygenase content in the crude extract of recombinant cells co-expressing the 10S-dioxygenase gene with pGro7 is higher than that of recombinant cells co-expressing pKJE7 or pG-KJE8. Production of 360 mg/g/h 10S-hydroxy-8(E)-octadecenoic acid from oleic acid