1.13.11.71: carotenoid-9',10'-cleaving dioxygenase

This is an abbreviated version!
For detailed information about carotenoid-9',10'-cleaving dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.71

Reaction

all-trans-beta-carotene
+
O2
=
all-trans-10'-apo-beta-carotenal
+
beta-ionone

Synonyms

BC-9',10'-oxygenase, Bcdo2, BCO2, beta,beta-carotene 9',10'-dioxygenase 2, beta,beta-carotene 9',10'-oxygenase, beta,beta-carotene-9',10'-dioxygenase, beta,beta-carotene-9,10-oxygenase 2, beta-carotene 9',10' oxygenase, beta-carotene 9',10'-oxygenase, beta-carotene-9',10'-oxygenase, beta-carotene-9,10'-oxygenase, CCD7, LjCCD7, ScBCO2, xanthophyll cleavage enzyme

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.71 carotenoid-9',10'-cleaving dioxygenase

Purification

Purification on EC 1.13.11.71 - carotenoid-9',10'-cleaving dioxygenase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
a small amount of recombinant, catalytically inactive His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by cobalt affinity chromatography
B2RCV8
native enzyme 9.5fold to homogeneity by ammonium sulfate fractionation and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by cobalt affinity chromatography