1.13.11.70: all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase

This is an abbreviated version!
For detailed information about all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase, go to the full flat file.

Reaction

all-trans-10'-apo-beta-carotenal
+
O2
=
13-apo-beta-carotenone
+
(2E,4E,6E)-4-methylocta-2,4,6-trienedial

Synonyms

carotenoid cleavage dioxygenase, CCD8, MAX4, MORE AXILLARY BRANCHING 4, NCED8, Rms1

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.70 all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase

Cloned

Cloned on EC 1.13.11.70 - all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene ccd8, quantitative real-time and RT-PCR enzyme expression analysis
-
gene ccd8, recombinant expression of N-terminally His6-tagged AtCCD8 lacking the first 168 bp, corresponding to a chloroplast transit peptide, in Escherichia coli strain BL21
gene ccd8, sequence comparisons