1.13.11.68: 9-cis-beta-carotene 9',10'-cleaving dioxygenase
This is an abbreviated version!
For detailed information about 9-cis-beta-carotene 9',10'-cleaving dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.68
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1.13.11.68
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strigolactones
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sls
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shoot
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phytohormone
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axillary
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dwarf27
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tillering
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carlactone
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weed
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carotenoid-derived
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apocarotenoids
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phelipanche
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ramosa
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orobanche
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arbuscular
- 1.13.11.68
- strigolactones
- sls
- shoot
-
phytohormone
-
axillary
- dwarf27
-
tillering
- carlactone
-
weed
-
carotenoid-derived
-
apocarotenoids
- phelipanche
- ramosa
- orobanche
-
arbuscular
Reaction
Synonyms
AtCCD7, carotenoid cleavage dioxygenase, carotenoid cleavage dioxygenase 7, carotenoid cleavage dioxygenases 7, CCD1, CCD4b, CCD7, DAD3, HTD1/D17, MAX3, MAX3/CCD7, NCED7, OsCCD7, PsCCD7, RMS5, SL biosynthetic enzyme, strigolactone biosynthetic enzyme, ZmCCD7, ZpCCD7
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Engineering
Engineering on EC 1.13.11.68 - 9-cis-beta-carotene 9',10'-cleaving dioxygenase
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CCD7M14
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the chickpea mutant with G-to-A transition at nucleotide position 210 leading to stop codon at position 70 of the protein shows considerable resistance to broomrape (high resistance to both Phelipanche aegyptiaca and Orobanche crenata) and a strigolactone-deficiency phenotype, with a high number of short branches compared to wild type
additional information
enzyme is able to complement a CCD7/max3 mutation in Arabidopsis thaliana
additional information
a truncated enzyme protein of 587 amino acids lacking the N-terminal targeting sequence is also able to catalyze the 910 cleavage of beta-carotene. Coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching
additional information
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gene silencing of CCD7 in Phelipanche aegyptiaca by tobacco rattle virus system, parasitic PaCCD7 gene is silenced in Phelipanche aegyptiaca using a trans-silencing approach in Nicotiana benthamiana. Transient knockdown of PaCCD7 inhibits tubercle development and the infestation process in host plants