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1.13.11.63: beta-carotene 15,15'-dioxygenase

This is an abbreviated version!
For detailed information about beta-carotene 15,15'-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.63

Reaction

beta-carotene
+
O2
= 2 all-trans retinal

Synonyms

BC-15,15'-oxygenase, BCM, BCMO, Bcmo1, Bcmo2, BCO, BCO1, BCO2, Bcox, beta,beta-carotene 15,15'-mono-oxygenase 1, beta,beta-carotene 15,15'-monooxygenase, beta,beta-carotene 15,15'-monooxygenase 1, beta,beta-carotene 15,15'-monooxygenase-1, beta,beta-carotene 15,15-monooxygenase 1, beta,beta-carotene-15,15'-monooxygenase, beta,beta-carotene-15,15'-oxygenase, beta-carotene 15,15' oxygenase, beta-carotene 15,15'-dioxygenase, beta-carotene 15,15'-monooxygenase, beta-carotene 15,15'-monooxygenase 1, beta-carotene 15,15'-oxygenase, beta-carotene 15,15-monooxygenase, beta-carotene dioxygenase 1, beta-carotene oxygenase 2, beta-carotene-15,15'-dioxygenase, beta-carotene-15,15'-monooxygenase, beta-carotene-15,15'-oxygenase, beta-carotene-15,15'-oxygenase-1, beta-carotene-15,15'-oxygenase-2, beta-CD, betaCDIOX, betaCO, BLH, brp/blh-family beta-carotene monooxygenase, brpA, carotenoid oxygenase, Ci-BCO, CMO1, EC 1.13.11.21, EC 1.14.99.36, NinaB

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.63 beta-carotene 15,15'-dioxygenase

Cloned

Cloned on EC 1.13.11.63 - beta-carotene 15,15'-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculovurus expressed
BCMO gene, DNA and mino acid sequence determination and analysis, expression in Escherichia coli strain ER2566
DNA and amino acid sequence determination and analysis, genotyping, two fully linked SNPs are identified within the proximal promoter of the BCMO1 gene at -678 and -621 upstream from the ATG codon corresponding to the substitution of two adenines (A/A) by two guanines (G/G) and defined two haplotypes, AN57A and GN57G, which segregate within chicken lines, quantitative PCR enzyme expression analysis
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EST library screening, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
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expressed in a xylose-fermenting Saccharomyces cerevisiae strain S8
expressed in Sf9 insect cells
expressed in Synechococcus sp. PCC 7002
expression by an baculovirus/Spodoptera frugiperda 9 insect cell system
expression in Escherichia coli
expression in Escherichia coli and in CHO cells
expression of BCO1 with a C-terminal polyhistidine tag in Escherichia coli strain BL21-Gold(DE3)
for expression in Escherichia coli cells, genetically engineered to produce beta-carotene
from intestinal cDNA library
-
gene bcmo1, DNA and amino acid sequence determination and analysis
gene BCMO1, expression of enzyme with C-terminal C-terminal TEV-, His6- and 1D4-tags in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method
gene BCMO1, overlapping binding sites for HNF-1alpha and HNF-4alpha in the human BCMO1 gene proximal promoter. Endogenous nuclear HNF-1alpha and HNF-4alpha proteins competitively bind these sites in Caco-2 BBe cells. BCMO1 gene expression levels and promoter activity are significantly decreased in HNF-1alpha siRNA-transfected Caco-2 BBe cells. In contrast, HNF-4alpha siRNA-transfected cells exhibit a significant increase in BCMO1 gene expression and promoter activity. Mutation of these overlapping binding sites dramatically decreases BCMO1 promoter activity. Quantitative real-time reverse transcription-PCR enzyme expression analysis and BCMO1 promoter activity analysis
gene bco1, quantitative PCR enzyme expression analysis
gene bco1, quantitative real-time PCR enzyme expression analysis
gene bco2, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, genotyping, overview
gene brpA, encoding a a membrane protein with homology to a brp/blh-family beta-carotene monooxygenase, DNA and amino acid sequence determination and analysis, sequence comparisons, genotyping, cloning and heterologous expression of gene brpA in Escherichia coli confers a significant salt tolerance phenotype. When cultured in the presence of exogenous beta-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane
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identification of ligand binding sites on the bcmo1 promoter, transcriptional regulation, overview
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into a Flag fusion-modified mammalian expression vector pcDNA3 and into the bacterial vectors pET-15b and pGEX-4T2
into the vector pET-24a+ for expression in Escherichia coli ER2566 cells
into the vector pET-24b+ for expression in Escherichia coli ER2566 cells
into the vectors pCRII TOPO and pBAD-TOPO for expression in Escherichia coli XL1-blue cells
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isozyme Bcmo1, quantitative real-time PCR expression analysis
isozyme Bcmo2, quantitative real-time PCR expression analysis
quantitative reverse-transcriptase PCR enzyme expression analysis
real-time quantitative RT-PCR enzyme expression analysis
tissue-specific quantitative RT-PCR expression analysis
-