1.13.11.60: linoleate 8R-lipoxygenase
This is an abbreviated version!
For detailed information about linoleate 8R-lipoxygenase, go to the full flat file.
Word Map on EC 1.13.11.60
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1.13.11.60
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hydroperoxide
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graminis
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gaeumannomyces
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oxylipins
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dioxygenation
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antarafacial
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8r-hydroperoxylinoleic
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allene
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aspergilli
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suprafacial
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cyclooxygenases
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ppoas
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verticillioides
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take-all
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homolytic
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zymoseptoria
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ferryl
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synthesis
- 1.13.11.60
- hydroperoxide
- graminis
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gaeumannomyces
- oxylipins
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dioxygenation
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antarafacial
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8r-hydroperoxylinoleic
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allene
-
aspergilli
-
suprafacial
-
cyclooxygenases
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ppoas
- verticillioides
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take-all
-
homolytic
-
zymoseptoria
-
ferryl
- synthesis
Reaction
Synonyms
5,8-LDS, 5,8-linoleate diol synthase, 6,8-diol synthase, 6R,8R-linoleate diol synthase, 6R,8RLDS, 7,8-diol synthase, 7,8-LDS, 7,8-linoleate diol synthase, 8(R)-dioxygenase, 8,11-hydroperoxide isomerase, 8-DOX, 8R-dioxygenase, 8R-DOX, 8R-DOX-5,8-LDS, 8R-DOX-7,8-LDS, 8R-DOX-LDS, dioxygenase-cytochrome P450, DOX-CYP, EC 1.13.11.44, EGE82165, FOXB_09952, GLRG_10013, linoleate 7,8-LDS, linoleate 8,11-LDS, linoleate diol synthase, linoleic acid 8R-dioxygenase, ODH51007, orphan DOX-CYP, PpoA
ECTree
1.13.11.60 linoleate 8R-lipoxygenaseAdvanced search results
results (
results (Inhibitors
Inhibitors on EC 1.13.11.60 - linoleate 8R-lipoxygenase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
linoleic acid
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0.1 mM, partial inactivation, may indicate product inhibition
N-tosyl-L-phenylalanyl chloromethyl ketone
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1 mM, over 70% inhibition
phenylmethanesulfonyl fluoride
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0.2 mM, inhibits the 8R-dioxygenase enzyme by over 85%
N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid
i.e. BW A4C, more than 90% inhibition at 0.01 mM, about 50% inhibition at 0.001 mM
N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid
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i.e. BW A4C
additional information
suicide inactivation, activity falls to zero within a few min although substrates were available in sufficient amounts
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additional information
not inhibited by 1 mM KCN. CO does not inhibit the enzyme
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additional information
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catalysis declines due to suicide inactivation; catalysis declines due to suicide inactivation. Following column chromatography, e.g. ion exchange or zinc-affinity chromatography, hydroperoxide isomerase activity is often decreased and sometimes even undetectable by TLC, whereas 8-dioxygenase activity is always present. However, storage of the enzyme for a few days on ice often restores the hydroperoxide isomerase and appeares to increase the 7,8-linoleate diol synthase activity as well
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additional information
catalysis declines due to suicide inactivation; catalysis declines due to suicide inactivation. Following column chromatography, e.g. ion exchange or zinc-affinity chromatography, hydroperoxide isomerase activity is often decreased and sometimes even undetectable by TLC, whereas 8-dioxygenase activity is always present. However, storage of the enzyme for a few days on ice often restores the hydroperoxide isomerase and appeares to increase the 7,8-linoleate diol synthase activity as well
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additional information
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GSH (1 mM), GSSG (1 mM), EDTA (1 or 10 mM), EGTA (1 or 10 mM) and H202 (10 mM) are without effect on the 8R-dioxygenase
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additional information
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sorbitol (0.75 M, 14%), glycerol (15%) and ethylene glycol (25%) do not inhibit the enzyme
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