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1.13.11.52: indoleamine 2,3-dioxygenase

This is an abbreviated version!
For detailed information about indoleamine 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.52

Reaction

D-tryptophan
+
O2
=
N-formyl-D-kynurenine

Synonyms

31854, BRAFLDRAFT_126354, CG5163, EC 1.13.1.12, hIDO, hIDO1, hTDO, IDO, IDO-1, IDO-2, IDO-I, IDO-II, IDO-III, IDO-IV, IDO1, IDO2, INDO, INDOL1, indolamine 2,3-dioxygenase, indole 2,3-dioxygenase, indoleamine 2, 3-dioxygenase, indoleamine 2,3 dioxygenase, indoleamine 2,3-dioxygenase, indoleamine 2,3-dioxygenase 1, indoleamine 2,3-dioxygenase 2, indoleamine 2,3-dioxygenase-1, indoleamine 2,3-dioxygenase-2, indoleamine 2,3-dioxygenase-like protein, indoleamine-2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase, L-tryptophan 2,3-dioxygenase, L-tryptophan pyrrolase, mIDO, oxygenase, tryptophan 2,3-di-, proto-IDO, proto-indoleamine 2,3-dioxygenase, superoxygenase, TDO, TDO2, TioF, TO, TRPO, Tryptamin 2,3-dioxygenase, tryptamine 2,3-dioxygenase, tryptophan 2,3-dioxygenase, tryptophan dioxygenase, tryptophan oxygenase, tryptophan peroxidase, tryptophan pyrrolase, tryptophan-2,3-dioxygenase, tryptophanase, v1g244579, Vermilion protein

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.52 indoleamine 2,3-dioxygenase

Cloned

Cloned on EC 1.13.11.52 - indoleamine 2,3-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminal hexahistidine tag, expressed in Escherichia coli
-
cell-free transcription/translation using a wheat germ system, expression in COS-7 cell
-
construction of a cDNA clone and its use in isolating genomic clones containing the structural gene
-
construction of recombinant replication-defective adenoviruses harboring the human indoleamine 2,3-dioxygenase gene and expression in dendritic cells
-
COS-7 cells expressing active human IDO
-
DNA and amino acid sequence determination and analysis, sequence and genetic structure comparisons, and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain KRX. Complementation of the enzyme-deficient Saccharomyces cerevisiae
DNA and amino acid sequence determination and analysis, sequence and genetic structure comparisons, and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain KRX. No complementation of the enzyme-deficient Saccharomyces cerevisiae
-
enzyme with a His tag at the N-terminus is expressed in Escherichia coli JM109 harboring plasmid pUC18 carrying the full-length cDNA of TDO
-
expressed in COS-7 and HEK293 cell, C-terminal hemagglutinin-tagged
expressed in Escherichia coli
expressed in Escherichia coli BL21 AI cells
expressed in Escherichia coli BL21 Star cells
expressed in HEK-293T cell
-
expressed in HeLa cells
-
expressed in Mus musculus
-
expression in COS-7 cells
expression in Escherichia coli
expression in Escherichia coli as a full-length, C-terminal hexahistidine fusion protein
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli E538, expressed with His-Tag
-
expression in Escherichia coli E538, formation of inclusion bodies at 37°C with reduced formation, at 30°C, expressed with His-Tag
-
expression in Escherichia coli EC538 as a fusion protein to a hexahistidyl tag
-
expression in Escherichia coli strain YA21
-
expression in Saccharomyces cerevisiae tryptophan auxotroph can restore growth in presence of low tryptophan concentrations
-
expression in Sf9 cells
expression in the Escherichia coli KRX strain
expression of Ag-HisTDO in Escherichia coli
-
expression of His-tagged enzyme in Escherichia coli, optimised expression
-
expression of His6-tagged IDO1 in Escherichia coli strain BL21 Star
F5H5G0, P14902
expression of His6-tagged IDO2 in Escherichia coli strain BL21 Star
F5H5G0, P14902
expression of His6-tagged TDO in Escherichia coli strain BL21 (DE3)
expression of recombinant His-tagged enzyme
-
full-length enzyme is cloned into a pET-21d derivative, with a C-terminal hexahistidine tag, and overexpressed at 17°C in Escherichia coli BL21(DE3) pMGK cells
gene 31854, DNA and amino acid sequence determination and analysis, sequence and genetic structure comparisons, and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain KRX. Slight complementation of the enzyme-deficient Saccharomyces cerevisiae
gene BRAFLDRAFT_126354, DNA and amino acid sequence determination and analysis, sequence and genetic structure comparisons, and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain KRX. Slight complementation of the enzyme-deficient Saccharomyces cerevisiae
gene IDO1, DNA and amino acid sequence determination and analysis, sequence and genetic structure comparisons, and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain KRX. No complementation of the enzyme-deficient Saccharomyces cerevisiae
gene IDO1, recombinant expression His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene IDO1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star (DE3), real-time PCR enzyme expression analysis
gene IDO1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene IDO1, semi-quantitative RT-PCR and quantitative real-time RT-PCR enzyme expression analysis in lipopolysaccgaride-stimulated THP-1 cells, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli
gene Ido2, DNA and amino acid sequence determination and analysis, sequence and genetic structure comparisons, and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain KRX. No complementation of the enzyme-deficient Saccharomyces cerevisiae
gene IDO2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene iso1, DNA and amino acid sequence determination and analysis, sequence and genetic structure comparisons, and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain KRX. No complementation of the enzyme-deficient Saccharomyces cerevisiae
gene v1g244579, DNA and amino acid sequence determination and analysis, sequence and genetic structure comparisons, and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain KRX. No complementation of the enzyme-deficient Saccharomyces cerevisiae
GST-fusioned protein, expressed in Escherichia coli, expressed in HEK-293T cell
hexa-histidine tag, expressed in Escherichia coli
-
hexahistidine tag, expressed in Escherichia coli BL21
hexahistidyl tagged, expressed in Escherichia coli, expressed in HEK-293T cell
isozyme IDO2 is expressed in KRX cells, isozyme IDO1 is expressed in Escherichia coli Rosetta cells
-
large scale expression in Escherichia coli
N-terminal His tag, expressed in Escherichia coli
-
quantitative real-time RT PCR enzyme expression analysis
recombinant expression in Escherichia coli
-
recombinant overexpression of enzyme IDO in HEK-293 cells