1.13.11.50: acetylacetone-cleaving enzyme

This is an abbreviated version!
For detailed information about acetylacetone-cleaving enzyme, go to the full flat file.

Reaction

Synonyms

acetylacetone dioxygenase, acetylacetone-cleaving enzyme, b-diketone dioxygenase, beta-diketone dioxygenase, cupin-type dioxygenase, diketone cleaving dioxygenase, diketone cleaving enzyme, diketone dioxygenase, diketone-cleaving dioxygenase, diketone-cleaving enzyme, DKDO, Dke1, oxygenase, b-diketone di-, pentane-2,4-dione hydrolase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.50 acetylacetone-cleaving enzyme

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
14	AA Sequence
1	Application
1	CAS Registry Number
7	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
3	General Information
1	General Stability
11	Inhibitors
15	KM Value [mM]
1	Localization
22	Metals/Ions
11	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
15	Organism
4	PDB ID
4	pH Optimum
2	pH Range
25	Protein Variants
6	Purification (Commentary)
4	Reaction
5	Reaction Type
15	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
45	Substrates and Products (Substrate)
6	Subunits
35	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
31	Turnover Number [1/s]

Turnover Number

print visible entries

print all entries

Turnover Number on EC 1.13.11.50 - acetylacetone-cleaving enzyme

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Turnover Number Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0049

1,1,1-trifluoro-2,4-pentanedione

Acinetobacter johnsonii

-

apparent value at 25°C

674150

0.0043 - 6.6

1,1,1-trifluoro-pentane-2,4-dione

11 entries

0.036

1,1-difluoro-2,4-pentanedione

Acinetobacter johnsonii

-

apparent value at 25°C

674150

0.0015 - 0.036

1,1-difluoropentane-2,4-dione

5 entries

0.00043

4,4-difluoro-1-phenyl-1,3-butanedione

Acinetobacter johnsonii

-

apparent value at 25°C

674150

8.5

acetylacetate

Acinetobacter johnsonii

-

-

657866

0.7 - 8.5

Pentane-2,4-dione

11 entries

0.0043

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

-

native enzyme, using 0.09 mM 1,1,1-trifluoro-pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C

675466

0.007

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant Y70A/R80A/E98A

711267

0.033

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant Y70F/R80A/E98A

711267

0.05

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant E98Q

711267

0.074

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant E98A

711267

0.098

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant R80A/E98A

711267

0.1

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant T107A

711267

0.126

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant R80A

711267

0.13

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant Y70A

711267

1.27

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant mutant Y70F

711267

6.6

1,1,1-trifluoro-pentane-2,4-dione

Acinetobacter johnsonii

Q8GNT2

pH 7.5, 25°C, recombinant wild-type enzyme

711267

0.0015

1,1-difluoropentane-2,4-dione

Acinetobacter johnsonii

-

mutant Y70F, pH 7.5, 25°C

725553

0.008

1,1-difluoropentane-2,4-dione

Acinetobacter johnsonii

-

mutant F59A, pH 7.5, 25°C

725553

0.018

1,1-difluoropentane-2,4-dione

Acinetobacter johnsonii

-

mutant F115A, pH 7.5, 25°C

725553

0.022

1,1-difluoropentane-2,4-dione

Acinetobacter johnsonii

-

mutant F119A, pH 7.5, 25°C

725553

0.036

1,1-difluoropentane-2,4-dione

Acinetobacter johnsonii

-

wild-type enzyme, pH 7.5, 25°C

725553

0.7

Pentane-2,4-dione

Acinetobacter johnsonii

-

mutant F59A, pH 7.5, 25°C

725553

1.27

Pentane-2,4-dione

Acinetobacter johnsonii

-

mutant Y70F, pH 7.5, 25°C

725553

1.4

Pentane-2,4-dione

Acinetobacter johnsonii

-

mutant F115A, pH 7.5, 25°C

725553

3.2

Pentane-2,4-dione

Acinetobacter johnsonii

-

mutant F119A, pH 7.5, 25°C

725553

3.6

Pentane-2,4-dione

Acinetobacter johnsonii

-

mutant enzyme E69Q, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C

675466

6.5

Pentane-2,4-dione

Acinetobacter johnsonii

-

apparent value at 25°C

674150

6.6

Pentane-2,4-dione

Acinetobacter johnsonii

-

wild-type enzyme, pH 7.5, 25°C

725553

6.6

Pentane-2,4-dione

Acinetobacter johnsonii

-

Strep-tagged enzyme

671683

6.6

Pentane-2,4-dione

Acinetobacter johnsonii

-

wild type enzyme, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C

675466

7

Pentane-2,4-dione

Acinetobacter johnsonii

-

recombinant enzyme

671683

8.5

Pentane-2,4-dione

Acinetobacter johnsonii

-

native enzyme

671683