1.13.11.50: acetylacetone-cleaving enzyme

This is an abbreviated version, for detailed information about acetylacetone-cleaving enzyme, go to the full flat file.

Reaction

Pentane-2,4-dione
+
O2
=
acetate
+
methylglyoxal

Synonyms

acetylacetone dioxygenase, acetylacetone-cleaving enzyme, b-diketone dioxygenase, cupin-type dioxygenase, diketone cleaving dioxygenase, diketone cleaving enzyme, diketone dioxygenase, diketone-cleaving dioxygenase, diketone-cleaving enzyme, Dke1, oxygenase, b-diketone di-, pentane-2,4-dione hydrolase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.50 acetylacetone-cleaving enzyme

Purification

Purification on EC 1.13.11.50 - acetylacetone-cleaving enzyme

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity column chromatography
-
purified, concentrated enzyme is dialyzed twice against 2 mM EDTA in 20 mM Tris/HCl (pH 7.5) to strip off iron, incubation in 2 mM metal ion as sulfate salt plus ascorbate to prevent Fe2+ oxidation, unbound metal ions are removed by 3 cycles of gel filtration using nucleic acid purification column
-
recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography
-
Sepharose Q fast flow column chromatography Superdex 200 gel filtration, Resource Q column chromatography, Nap-5 column gel filtration, and Phenyl Sepharose HP chromatography
-