1.13.11.50: acetylacetone-cleaving enzyme

This is an abbreviated version!
For detailed information about acetylacetone-cleaving enzyme, go to the full flat file.

Word Map on EC 1.13.11.50

Reaction

Pentane-2,4-dione
+
O2
=
acetate
+
2-oxopropanal

Synonyms

acetylacetone dioxygenase, acetylacetone-cleaving enzyme, b-diketone dioxygenase, cupin-type dioxygenase, diketone cleaving dioxygenase, diketone cleaving enzyme, diketone dioxygenase, diketone-cleaving dioxygenase, diketone-cleaving enzyme, Dke1, oxygenase, b-diketone di-, pentane-2,4-dione hydrolase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.50 acetylacetone-cleaving enzyme

Inhibitors

Inhibitors on EC 1.13.11.50 - acetylacetone-cleaving enzyme

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
20 mM Tris/HCl buffer, pH 7.5, 25°C, 1.2fold molar excess, reversible inactivation of wild-type and mutant enyzme through competition with Fe2+, substrates 200 microM pentane-2,4-dione, 330 microM quercetin, 330 microM potassium oxalate, 330 microM 3,4-dihydroxyphenylacetate
EDTA
-
largely irreversible losses
hexacyanoferrate(III)
-
2.5 mM, 80% decrease in activity within 10 min
KCN
-
largely irreversible losses
Mn2+
-
20 mM Tris/HCl buffer, pH 7.5, 25°C, 1.2fold molar excess, reversible inactivation of wild-type and mutant enyzme through competition with Fe2+, substrates 200 microM pentane-2,4-dione, 330 microM quercetin, 330 microM potassium oxalate, 330 microM 3,4-dihydroxyphenylacetate
Ni2+
-
20 mM Tris/HCl buffer, pH 7.5, 25°C, 1.2fold molar excess, reversible inactivation of wild-type and mutant enyzme through competition with Fe2+, substrates 200 microM pentane-2,4-dione, 330 microM quercetin, 330 microM potassium oxalate, 330 microM 3,4-dihydroxyphenylacetate
o-phenanthroline
-
largely irreversible losses
Zn2+
-
20 mM Tris/HCl buffer, pH 7.5, 25°C, 1.2fold molar excess, reversible inactivation of wild-type and mutant enzyme through competition with Fe2+, substrates 200 microM pentane-2,4-dione, 330 microM quercetin, 330 microM potassium oxalate, 330 microM 3,4-dihydroxyphenylacetate
additional information
-
no inactivation effect of Fe3+
-