1.13.11.5: homogentisate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about homogentisate 1,2-dioxygenase, go to the full flat file.

Reaction

Synonyms

EC 1.13.1.5, EC 1.99.2.5, ElHDO, HgD, HGDO, HGO, HGOa, HGOb, HmgA, homogentisate 1,2 dioxigenase, homogentisate 1,2 dioxygenase, homogentisate 1,2-dioxygenase, homogentisate dioxygenase, homogentisate oxidase, homogentisate oxygenase, homogentisate phytyl-transferase, homogentisic acid 1,2-dioxygenase, homogentisic acid oxidase, homogentisic acid oxygenase, homogentisic oxygenase, homogentisicase, HPT, HTO

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.5 homogentisate 1,2-dioxygenase

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Results	in table
16	Activating Compound
11185	AA Sequence
8	Application
1	CAS Registry Number
15	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
392	Disease/ Diagnostics
4	Expression
27	General Information
5	General Stability
41	Inhibitors
2	kcat/KM [mM/s]
14	KM Value [mM]
2	Localization
19	Metals/Ions
18	Molecular Weight [Da]
39	Natural Substrates/ Products (Substrates)
84	Organism
3	Oxidation Stability
7	Pathway
38	PDB ID
7	pH Optimum
1	pH Range
1	pH Stability
2	pI Value
1	Posttranslational Modification
58	Protein Variants
27	Purification (Commentary)
4	Reaction
3	Reaction Type
75	Reference
65	Source Tissue
28	Specific Activity [micromol/min/mg]
5	Storage Stability
84	Substrates and Products (Substrate)
14	Subunits
60	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
4	Temperature Stability [°C]
9	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.13.11.5 - homogentisate 1,2-dioxygenase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 50 mM potassium phosphate buffer, pH 7.0, crude extracts, 5 months, 25% loss of activity

Aspergillus nidulans

-

439389

-20°C, in absence of Fe2+, about 3 days, stable

Aspergillus niger

-

439386

-20°C, pH 6.0-7.2, in absence of Fe2+, several weeks, stable

Pseudomonas fluorescens

-

439380

-80, 20 mM Hepps, pH 8.0, 100 mM NaCl, 4 months, no loss of activity

Homo sapiens

-

657859

4°C, 20 mM Tris-Cl pH 7.8, 0.5 M NaCl, 0.3 M imidazole, 24 h, loses two third of its activity

Arabidopsis thaliana

Q9ZRA2

670622