1.13.11.41: 2,4'-dihydroxyacetophenone dioxygenase
This is an abbreviated version!
For detailed information about 2,4'-dihydroxyacetophenone dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.41
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1.13.11.41
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cupin
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alcaligenes
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homotetramer
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formic
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4-hydroxybenzoic
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ketone
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chymotrypsinolysis
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tridentate
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biomimetic
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nonhaem
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non-heme
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2-hydroxyacetophenone
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equiv
- 1.13.11.41
-
cupin
- alcaligenes
-
homotetramer
-
formic
-
4-hydroxybenzoic
- ketone
-
chymotrypsinolysis
-
tridentate
-
biomimetic
-
nonhaem
-
non-heme
- 2-hydroxyacetophenone
-
equiv
Reaction
Synonyms
(4-hydroxybenzoyl)methanol oxygenase, DAD, DHAP dioxygenase
ECTree
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Crystallization
Crystallization on EC 1.13.11.41 - 2,4'-dihydroxyacetophenone dioxygenase
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hanging drop vapour diffusion method, mixing of 5 mg/ml protein in 50 mM Tris, pH 7.3, 100 mM NaCl, and mM 4-hydroxybenzoic acid or 0.1 mM 4-aminobenzamidine, with reservoir solution containing 0.1 M sodium cacodylate pH 5.9-6.8 and 1.1-1.6 M sodium acetate, method optimization, X-ray diffraction structure determination and analysis at 1.88 A resolution. The use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, is necessary for crystallization of the protein
purified recombinant detagged chymotrypsinolysed enzyme, 5 mg/ml protein in 50 mM Tris, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol is mixed with a solution containing 10% w/v PEG 1000 and 10% PEG 8000, X-ray diffraction structure determination and analysis at 2.2 A resolution. The use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, is necessary for crystallization of the protein
purified recombinant His-tagged chymotrypsinolysed enzyme, 5 mg/ml protein in 50 mM Tris, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol is mixed with a solution containing 10% w/v PEG 1000 and 10% PEG 8000, X-ray diffraction structure determination and analysis at 2.2 A resolution. The use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, is necessary for crystallization of the protein