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1.13.11.38: 1-hydroxy-2-naphthoate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about 1-hydroxy-2-naphthoate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.38

Reaction

1-hydroxy-2-naphthoate
+
O2
=
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate

Synonyms

1-H2NA dioxygenase, 1-HNDO, 1-hydroxy-2-naphthoate 1,2-dioxygenase, 1-hydroxy-2-naphthoate dioxygenase, 1-hydroxy-2-naphthoate-degrading enzyme, 1-hydroxy-2-naphthoic acid dioxygenase, 1H2NDO, Diox1, Diox2, oxygenase, 1-hydroxy-2-naphthoate di-, Sphe3 1-H2NA dioxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.38 1-hydroxy-2-naphthoate 1,2-dioxygenase

Purification

Purification on EC 1.13.11.38 - 1-hydroxy-2-naphthoate 1,2-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme by anion exchange and hydrophobic interaction chromatography, and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
strain B156, isolated from soil and able to grow on phenanthrene as sole source of carbon and energy, partial purification
Gram-negative coccus
-
to homogeneity using a 3-hydroxy-2-naphthoic acid (3-H2NA)-affinity matrix, DEAE-anion-exchange chromatography
-