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0.027
(4-hydroxyphenyl)-pyruvate
-
pH 7.0, 25°C
0.5
2-thienylpyruvate
-
-
0.05
3,4-Dihydroxyphenylpyruvate
-
-
0.25
3-thienylpyruvate
-
-
0.0017 - 54
4-hydroxyphenylpyruvate
0.05
4-hydroxytetrafluorophenylpyruvate
-
-
0.115
oxygen
-
activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C
0.06 - 0.52
phenylpyruvate
additional information
additional information
-
0.0017
4-hydroxyphenylpyruvate
-
mutant V218L/A327R/I340E/L359M , pH 7, 25°C
0.00187
4-hydroxyphenylpyruvate
wild-type, pH 7, 25°C
0.002
4-hydroxyphenylpyruvate
-
mutant V218I/A327R/I340E/L359M, pH 7, 25°C
0.0026
4-hydroxyphenylpyruvate
-
mutant A327R/L359M, pH 7, 25°C
0.00304
4-hydroxyphenylpyruvate
pH 7, 25°C
0.0031
4-hydroxyphenylpyruvate
-
mutant V218I/A327R/L359M, pH 7, 25°C
0.0043
4-hydroxyphenylpyruvate
-
-
0.0043
4-hydroxyphenylpyruvate
-
mutant L359M, pH 7, 25°C
0.0048
4-hydroxyphenylpyruvate
-
wild-type, pH 7, 25°C
0.0053
4-hydroxyphenylpyruvate
mutant S267A, pH 7, 25°C
0.0069
4-hydroxyphenylpyruvate
-
activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C
0.0075
4-hydroxyphenylpyruvate
-
recombinant wild-type enzyme, pH and temperature not specified in the publication
0.0087
4-hydroxyphenylpyruvate
-
pH 7.0, 25°C, mutant P243T
0.009
4-hydroxyphenylpyruvate
-
recombinant mutant S246A, pH and temperature not specified in the publication
0.0091
4-hydroxyphenylpyruvate
mutant F381A, pH 7, 25°C
0.0108
4-hydroxyphenylpyruvate
mutant N282A, pH 7, 25°C
0.0143
4-hydroxyphenylpyruvate
-
mutant V218I/A327R/I340E/L359M/G411A, pH 7, 25°C
0.015
4-hydroxyphenylpyruvate
-
pH 7.0, 25°C, mutant N245Q
0.0157
4-hydroxyphenylpyruvate
mutant N423A, pH 7, 25°C
0.025
4-hydroxyphenylpyruvate
-
enzyme form 1
0.027
4-hydroxyphenylpyruvate
-
enzyme form 2
0.027
4-hydroxyphenylpyruvate
-
pH 7.0, 25°C, wild-type enzyme and mutant N245I
0.029
4-hydroxyphenylpyruvate
-
enzyme form 3
0.03
4-hydroxyphenylpyruvate
-
-
0.03
4-hydroxyphenylpyruvate
-
-
0.0303
4-hydroxyphenylpyruvate
mutant Q307A, pH 7, 25°C
0.0309
4-hydroxyphenylpyruvate
-
mutant G413W, pH 7, 25°C
0.0389
4-hydroxyphenylpyruvate
-
mutant G411A, pH 7, 25°C
0.04
4-hydroxyphenylpyruvate
-
-
0.04
4-hydroxyphenylpyruvate
-
-
0.041
4-hydroxyphenylpyruvate
-
-
0.0432
4-hydroxyphenylpyruvate
-
0.047
4-hydroxyphenylpyruvate
-
pH 7.0, 25°C, mutant S230A
0.05
4-hydroxyphenylpyruvate
-
-
0.07
4-hydroxyphenylpyruvate
pH 6.5, 37°C, mutant DELTAL381
0.075
4-hydroxyphenylpyruvate
-
-
0.077
4-hydroxyphenylpyruvate
wild-type, pH 7,20°C
0.08
4-hydroxyphenylpyruvate
pH 6.5, 37°C, wild-type enzyme and mutant DELTAG388
0.083
4-hydroxyphenylpyruvate
pH 6, 35°C
0.09
4-hydroxyphenylpyruvate
pH 6.5, 37°C, mutant DELTAL385
0.0904
4-hydroxyphenylpyruvate
mutant Q293A, pH 7, 25°C
0.098
4-hydroxyphenylpyruvate
mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7,20°C
0.1
4-hydroxyphenylpyruvate
mutant K10A, pH 6.5, 37°C
0.1
4-hydroxyphenylpyruvate
mutant lacking 17 N-terminal residues, pH 6.5, 37°C
0.11
4-hydroxyphenylpyruvate
pH 6.5, 37°C, mutant E254D
0.12
4-hydroxyphenylpyruvate
pH 6.5, 37°C, mutant DELTAG379
0.134
4-hydroxyphenylpyruvate
mutant M335H/P336A/E363Q, pH 7,20°C
0.142
4-hydroxyphenylpyruvate
pH 7.0, 50°C
0.153
4-hydroxyphenylpyruvate
-
recombinant mutant N261D, pH and temperature not specified in the publication
0.18
4-hydroxyphenylpyruvate
mutant E12A, pH 6.5, 37°C
0.18
4-hydroxyphenylpyruvate
mutant P11A, pH 6.5, 37°C
0.2
4-hydroxyphenylpyruvate
wild-type, pH 6.5, 37°C
0.2
4-hydroxyphenylpyruvate
mutant Q334N, pH 6.5, 37°C
0.24
4-hydroxyphenylpyruvate
wild-type, pH 6.5, 37°C
0.24
4-hydroxyphenylpyruvate
pH 6.5, 37°C, mutant R378K
0.286
4-hydroxyphenylpyruvate
-
recombinant mutant Q358E, pH and temperature not specified in the publication
0.3
4-hydroxyphenylpyruvate
pH 6.5, 37°C, mutant DELTAN380
0.3
4-hydroxyphenylpyruvate
mutant N363A, pH 6.5, 37°C
0.3
4-hydroxyphenylpyruvate
mutant Q334A, pH 6.5, 37°C
0.32
4-hydroxyphenylpyruvate
-
pH 7,20°C
0.37
4-hydroxyphenylpyruvate
mutant lacking 12 N-terminal residues, pH 6.5, 37°C
0.4
4-hydroxyphenylpyruvate
mutant N363Q, pH 6.5, 37°C
0.452
4-hydroxyphenylpyruvate
-
recombinant mutant Q272E, pH and temperature not specified in the publication
0.5
4-hydroxyphenylpyruvate
Frog
-
-
0.5
4-hydroxyphenylpyruvate
mutant N363D, pH 6.5, 37°C
0.5
4-hydroxyphenylpyruvate
mutant Q334A/N363D, pH 6.5, 37°C
0.5
4-hydroxyphenylpyruvate
mutant Q334N/N363Q, pH 6.5, 37°C
0.551
4-hydroxyphenylpyruvate
-
recombinant mutant Q286E, pH and temperature not specified in the publication
0.6
4-hydroxyphenylpyruvate
mutant Q334A/N363Q, pH 6.5, 37°C
0.9
4-hydroxyphenylpyruvate
mutant Q265E, pH 6.5, 37°C
11
4-hydroxyphenylpyruvate
-
Solvent is H2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture include ferrous sulfate (10 microM), DTT (1mM), HPPD (500 nM), and HPP (0-1 mM) in 20 mM HEPES, pH (pD) 7.0, under conditions of atmospheric oxygen (350 microM at 5°C)
22
4-hydroxyphenylpyruvate
-
Solvent is H2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture include ferrous sulfate (10 microM), DTT (1mM), HPPD (500 nM), and HPP (0-1 mM) in 20 mM HEPES, pH (pD) 7.0, under conditions of atmospheric oxygen (350 microM at 5°C)
54
4-hydroxyphenylpyruvate
-
Solvent is D2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture include ferrous sulfate (10 microM), DTT (1mM), HPPD (500 nM), and HPP (0-1 mM) in 20 mM HEPES, pH (pD) 7.0, under conditions of atmospheric oxygen (350 microM at 5°C)
0.05
O2
-
-
0.06
phenylpyruvate
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
the Km-value is the same at 25°C and at 38°C
-
additional information
additional information
-
the Km-value is the same at 25°C and at 38°C
-
additional information
additional information
-
Kd value for 4-hydroxyphenylpyruvate with 4-hydroxyphenylpyruvate dioxygenase Fe2+ is 29 microM, whereas the binding constant for phenylpyruvate is determined to be 319 microM
-