1.13.11.24: quercetin 2,3-dioxygenase
This is an abbreviated version!
For detailed information about quercetin 2,3-dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.24
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1.13.11.24
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flavonols
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dioxygenation
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bicupins
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o-heterocycle
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oxygenolysis
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synthesis
- 1.13.11.24
- flavonols
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dioxygenation
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bicupins
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o-heterocycle
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oxygenolysis
- synthesis
Reaction
Synonyms
2,3-QD, 2,3QD, 2,4-QD, Co-QDO, Co-QueD, Cu2+-containing 2,4-QD, cupin domain-containing protein, Fe-QDO, Fe-QueD, flavonol 2,4-dioxygenase, flavonol 2,4-oxygenase, manganese quercetin 2,3-dioxygenase, manganese quercetin dioxygenase, Mn-QDO, Mn-QueD, Ni-QueD, nickel quercetinase, pirin, QDO, QdoI, QueD, quercetin 2,4-dioxygenase, quercetin dioxygenase, quercetinase, type III extradiol dioxygenase, VdQase, YxaG
ECTree
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Purification
Purification on EC 1.13.11.24 - quercetin 2,3-dioxygenase
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DEAE-Sephacel column equilibrated with 50 mM Tris.HCl, pH 7.5, and eluted with a NaCl gradient (0-600 mM). Ultrogel ACA 34 column, eluted with 50 mM Tris.HCl, pH 7.5, and 100 mM Nacl. DEAE-Sepharose column equilibrated with 50 mM Tris.HCl, pH 7,5, eluted with a gradient of NaCl (100-500 mM).
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partial purification by ammonium sulfate precipitation and DEAE Toyopearl column chromatography
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recombinant C-terminally Strep-tagged wild-type and mutant enzymes from Escherichia coli to electrophoretic homogeneity by hydrophobic interaction chromatography, dialysis, two different steps of anion exchange chromatography, dialysis and ultrafiltration