1.13.11.20: cysteine dioxygenase

This is an abbreviated version!
For detailed information about cysteine dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.20

Reaction

L-cysteine
+
O2
=
3-sulfinoalanine

Synonyms

3-mercaptopropionate dioxygenase, Arg-type CDO, BsCDO, CDO, CDO1, CDO2, CdoA, CdoB, cysteine dioxygenase, cysteine oxidase, Fe(II) cysteine dioxygenase, H16_A1614, H16_B1863, oxygenase, cysteine di-

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.20 cysteine dioxygenase

Cloned

Cloned on EC 1.13.11.20 - cysteine dioxygenase

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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
amplification by PCR and cloning into TA cloning vector, sequencing
-
cloned in plasmid pVP16 to produce the enzyme in Escherichia coli B834 pRARE2, an N-terminal fusion to a His-8-maltose binding protein
-
cloned into pGEX-6p-1 expression vector, expressed in Escherichia coli Bl21(DE3), glutathione S-transferase-fused CDO is purified and GTS tag is removed by cleavage with PreScission Protease
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cloning of the gene and comparison of the gene to the known rat and human genes, sequencing, profiling of the enzyme mRNA and protein levels in mouse tissues
-
expressed as a His-tag fusion protein in Escherichia coli BL21(DE3)pLysS
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expressed as His-tag fusion protein in Escherichia coli
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expressed in BL21(D3)pLysS cells containing the pET-14b/CDO-ORF plasmid
-
expressed in BL21(DE3) cells
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli strain BL21(DE3)
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expressed in Hep-G2 cells
-
expression in Escherichia coli and HepG2/C3A cell
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expression in Escherichia coli strain BL21(DE3)
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expression in HepG2/C3A cell
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gene cdo, expression as N-terminal maltose-binding protein fusion protein in Escherichia coli strain BL21(DE3)
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gene cdo, expression as N-terminal maltose-binding protein fusion protein in Escherichia coli strain BL21(DE3)RIPL
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gene cdo, expression of Thx-His6 -tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene cdoA, recombinant enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia cli strain DH5alpha
gene H16_A1614, located on chromosome 1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoB in Escherichia coli; gene H16_B1863, located on chromosome 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoA in Escherichia coli. Escherichia coli cells expressing CdoA can convert 3-mercaptopropionate to 3-sulfinopropionate
heterologously expressed in human HepG2/C3A cells
-
primary structure of the cDNA for liver enzyme, sequence determination
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recombinant expression of Strep-tagged enzyme
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recombinant expression of the thioredoxin-His6-tagged enzyme
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recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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recombinant expression of wild-type and mutant Strep-tagged enzyme
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subcloned into an expression vector, pESC-TRP, for Saccharomyces cerevisiae; subcloned into an expression vector, pESC-TRP, for Saccharomyces cerevisiae
the open reading frame of putative cysteine dioxygenase NP_390992(yubC, Bacillus subtilis) is cloned and overexpressed in Escherichia coli.
the open reading frame of putative cysteine dioxygenase NP_627257 (SCO30335, Streptomyces coelicolor A3(2))is cloned and overexpressed in Escherichia coli; the open reading frame of putative cysteine dioxygenase NP_629897 (SCO5772, Streptomyces coelicolor A3(2)) is cloned and overexpressed in Escherichia coli
O50490, Q9KZL0
the open reading frame of putative cysteine dioxygenase NP_832375(BC2617, Bacillus cereus ATCC 14579) is cloned and overexpressed in Escherichia coli