1.13.11.20: cysteine dioxygenase

This is an abbreviated version!
For detailed information about cysteine dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.20

Reaction

L-cysteine
+
O2
=
3-sulfinoalanine

Synonyms

3-mercaptopropionate dioxygenase, Arg-type CDO, BsCDO, CDO, CDO1, CDO2, CdoA, CdoB, cysteine dioxygenase, cysteine oxidase, Fe(II) cysteine dioxygenase, H16_A1614, H16_B1863, oxygenase, cysteine di-

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.20 cysteine dioxygenase

Cloned

Cloned on EC 1.13.11.20 - cysteine dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amplification by PCR and cloning into TA cloning vector, sequencing
-
cloned in plasmid pVP16 to produce the enzyme in Escherichia coli B834 pRARE2, an N-terminal fusion to a His-8-maltose binding protein
cloned into pGEX-6p-1 expression vector, expressed in Escherichia coli Bl21(DE3), glutathione S-transferase-fused CDO is purified and GTS tag is removed by cleavage with PreScission Protease
cloning of the gene and comparison of the gene to the known rat and human genes, sequencing, profiling of the enzyme mRNA and protein levels in mouse tissues
expressed as a His-tag fusion protein in Escherichia coli BL21(DE3)pLysS
-
expressed as His-tag fusion protein in Escherichia coli
-
expressed in BL21(D3)pLysS cells containing the pET-14b/CDO-ORF plasmid
-
expressed in BL21(DE3) cells
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli strain BL21(DE3)
-
expressed in Hep-G2 cells
-
expression in Escherichia coli and HepG2/C3A cell
expression in Escherichia coli strain BL21(DE3)
-
expression in HepG2/C3A cell
-
gene cdo, expression as N-terminal maltose-binding protein fusion protein in Escherichia coli strain BL21(DE3)
-
gene cdo, expression as N-terminal maltose-binding protein fusion protein in Escherichia coli strain BL21(DE3)RIPL
-
gene cdo, expression of Thx-His6 ´-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene cdoA, recombinant enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia cli strain DH5alpha
gene H16_A1614, located on chromosome 1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoB in Escherichia coli
gene H16_B1863, located on chromosome 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoA in Escherichia coli. Escherichia coli cells expressing CdoA can convert 3-mercaptopropionate to 3-sulfinopropionate
heterologously expressed in human HepG2/C3A cells
-
primary structure of the cDNA for liver enzyme, sequence determination
-
recombinant expression of Strep-tagged enzyme
recombinant expression of the thioredoxin-His6-tagged enzyme
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of wild-type and mutant Strep-tagged enzyme
subcloned into an expression vector, pESC-TRP, for Saccharomyces cerevisiae
the open reading frame of putative cysteine dioxygenase NP_390992(yubC, Bacillus subtilis) is cloned and overexpressed in Escherichia coli.
the open reading frame of putative cysteine dioxygenase NP_627257 (SCO30335, Streptomyces coelicolor A3(2))is cloned and overexpressed in Escherichia coli
the open reading frame of putative cysteine dioxygenase NP_629897 (SCO5772, Streptomyces coelicolor A3(2)) is cloned and overexpressed in Escherichia coli
the open reading frame of putative cysteine dioxygenase NP_832375(BC2617, Bacillus cereus ATCC 14579) is cloned and overexpressed in Escherichia coli