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1.13.11.12: linoleate 13S-lipoxygenase

This is an abbreviated version!
For detailed information about linoleate 13S-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.12

Reaction

alpha-linolenate
+
O2
=
(9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate

Synonyms

(13S)-lipoxygenase, 13-lipoxygenase, 13-LOX, 13S-lipoxygenase, 15-lipoxygenase-1, 15-LOX-1, 9/13-LOX, acidic lipoxygenase, Alox15, arachidonic acid 15S-lipoxygenating enzyme, AtLOX-2, AtLOX-3, AtLOX-4, AtLOX-6, CmLOX10, CmLOX13, iron 13S-lipoxygenase, liipoxygenase, linoleate 13-lipoxygenase, linoleate 13S-lipoxygenase, linoleate oxygen oxidoreductase, linoleate: oxygen oxidoreductase, linoleate:oxygen oxidoreductase, lipoxygenase, lipoxygenase 2, lipoxygenase-1, lipoxygenase-2, lipoxygenase-3, lipoxygenase-4, lipoxygenase-6, LO-1, LOX, LOX-1, LOX-2, LOX1, LOX10, LOX11, LOX12, LOX2, LOX2:Hv:1, LOX2:Md:2b, LOX2b, LOX3, LOX4, LOX6, LOX7, LOX8, LOX9, LoxA, Lpx-A2, MSD2, Oep1LOX2, Oep2LOX2, omega-6 linoleate lipoxygenase, PA-LOX, RCI-1, SBLO-1, secreted lipoxygenase, SLO, soybean lipoxygenase-1, StLOX2, StLOX3, type-1 LOX, type-2 13-LOX

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.12 linoleate 13S-lipoxygenase

Cloned

Cloned on EC 1.13.11.12 - linoleate 13S-lipoxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain ER2566
-
Escherichia coli BL21 cells carrying the plasmid PT7-7/L1 VTSLO-11
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS and Rosetta(DE3)pLysS cells
-
expressed in Escherichia coli BL21-AI (DE3) cells
expressed in Escherichia coli NiCo21(DE3) cells
expressed in tobacco leaves
-
expression in Escherichia coli
expression in insect cells
gene CmLOX10, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3)
gene CmLOX13, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged truncated enzyme in Escherichia coli strain BL21(DE3)
gene FoxLOX, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparison, recombinnat expression of His-tagged enzyme
-
gene LOX2b, RT-PCR and real-time quantitative PCR expression analysis, 22 putative LOX genes in apple, gene expression analysis throughout ripening reveals that only six LOXs are expressed in a ripening-dependent manner, overview. DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged or untagged LOX genes endoing for isozymes LOX1:Md:1a, L, X1:Md:1c, LOX2:Md:2a, and LOX2:Md:2b, containing an enterokinase cleavage recognition site in the first case, in Saccharomyces cerevisiae strain INVSc1
gene rci-1, DNA and amino acid sequence determination and analysis, realtime PCR expression analysis, the gene shows a cultivar/tissue specific expression
gene tomloxD, overexpression in transgenic tomato plants, introduction of the binary vector pLoxD into Agrobacterium tumefaciens LBA4404 by the freeze-thaw method, phenotype, overview
-
into a pcDNA3.1 vector and introduced into HCT-116 and HT-29 cells
-
isoenzyme Oep1LOX2 and Oep2LOX2
-
LOX1 ORF cloned into vector pET30a(+) for overexpression in Escherichia coli. LOX1-green GFP fusion construct, under the control of the cauliflower mosaic virus 35S promoter, expressed in Arabidopsis plants. Transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter
Oep1LOX2 and Oep2LOX2 ligated into vector pQE-80 L and overexpressed in Escherichia coli strain XL1-Blue
-
overexpression and secretion of extracellular enzyme in Escherichia coli strain Rosetta(DE3) using the enzyme's endogenous signal peptide
-
recombinant baculovirus expressing LOX2 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
recombinant baculovirus expressing LOX3 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
recombinant baculovirus expressing LOX4 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
recombinant baculovirus expressing LOX6 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
recombinant overexpression of His-tagged enzyme in Escherichia coli BL21(DE3)
-
vector pQE30-LOX expressed in Escherichia coli M15 cells